Low-Intensity Pulsed Ultrasound Accelerates Traumatic Vertebral Fracture Healing by Coupling Proliferation of Type H Microvessels

被引:18
作者
Wu, Suiyi [1 ]
Xu, Ximing [2 ]
Sun, Jingchuan [2 ]
Zhang, Yao [3 ]
Shi, Jiangang [2 ]
Xu, Tianming [4 ]
机构
[1] Second Mil Med Univ, Fac Naval Med, Shanghai, Peoples R China
[2] Second Mil Med Univ, Changzheng Hosp, Dept Spine Surg, 415 Fengyang Rd, Shanghai 200003, Peoples R China
[3] Second Mil Med Univ, Cadet Brigade, Shanghai, Peoples R China
[4] 455 Hosp Chinese Peoples Liberat Army, Shanghai, Peoples R China
关键词
animal studies; bone density; low-intensity pulsed ultrasound; osteoblasts; traumatic vertebral fracture; type H microvessels; THERAPEUTIC ULTRASOUND; BONE REPAIR; CLASSIFICATION; ANGIOGENESIS; OSTEOGENESIS; CELLS; MODEL;
D O I
10.1002/jum.14525
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
Objectives-Patients with traumatic vertebral fractures often have major associated postoperative morbidities such as healing failure and kyphosis. Low-intensity pulsed ultrasound (US) has been found to promote bone fracture healing. The objectives of our study were to determine whether low-intensity pulsed US could promote traumatic vertebral fracture healing and to explore its inner mechanisms. Methods-A rat model of traumatic vertebral fracture was created and treated with low-intensity pulsed US after surgery. At 4 weeks after surgery, radiographic, micro-computed tomography, and 3-dimensional reconstruction were used to assess the radiologic healing status; a histologic analysis was performed to evaluate the pathologic process and relationship between osteogenesis and type H microvessels. Results-Well-remodeled trabecular meshworks were found in the low-intensity pulsed US treatment group compared to the control group. Micro-computed tomography and 3-dimensional reconstruction revealed more and thicker trabeculae after low-intensity pulsed US treatment. Abundant chondrocytes, a newly formed bone marrow cavity, trabeculae, and microvessels were formed at the fracture sites. More osterix-positive osteoblasts were circling the newly formed bone meshwork and were situated at the interface of chondrocytes in the low-intensity pulsed US treatment group. Type H microvessels were spreading around the newly formed trabecula, bone marrow cavity, osteoblasts, and interface of chondrocytes, with a larger mean vascular density in the low-intensity pulsed US group. Conclusions-Low-intensity pulsed US could accelerate traumatic vertebral fracture healing by temporally and spatially increasing chondrogenesis and osteoblast-induced osteogenesis coupled with angiogenesis of type H microvessels in a rat model of traumatic vertebral fracture.
引用
收藏
页码:1733 / 1742
页数:10
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