共 50 条
Low-Intensity Pulsed Ultrasound Accelerates Traumatic Vertebral Fracture Healing by Coupling Proliferation of Type H Microvessels
被引:18
作者:
Wu, Suiyi
[1
]
Xu, Ximing
[2
]
Sun, Jingchuan
[2
]
Zhang, Yao
[3
]
Shi, Jiangang
[2
]
Xu, Tianming
[4
]
机构:
[1] Second Mil Med Univ, Fac Naval Med, Shanghai, Peoples R China
[2] Second Mil Med Univ, Changzheng Hosp, Dept Spine Surg, 415 Fengyang Rd, Shanghai 200003, Peoples R China
[3] Second Mil Med Univ, Cadet Brigade, Shanghai, Peoples R China
[4] 455 Hosp Chinese Peoples Liberat Army, Shanghai, Peoples R China
关键词:
animal studies;
bone density;
low-intensity pulsed ultrasound;
osteoblasts;
traumatic vertebral fracture;
type H microvessels;
THERAPEUTIC ULTRASOUND;
BONE REPAIR;
CLASSIFICATION;
ANGIOGENESIS;
OSTEOGENESIS;
CELLS;
MODEL;
D O I:
10.1002/jum.14525
中图分类号:
O42 [声学];
学科分类号:
070206 ;
082403 ;
摘要:
Objectives-Patients with traumatic vertebral fractures often have major associated postoperative morbidities such as healing failure and kyphosis. Low-intensity pulsed ultrasound (US) has been found to promote bone fracture healing. The objectives of our study were to determine whether low-intensity pulsed US could promote traumatic vertebral fracture healing and to explore its inner mechanisms. Methods-A rat model of traumatic vertebral fracture was created and treated with low-intensity pulsed US after surgery. At 4 weeks after surgery, radiographic, micro-computed tomography, and 3-dimensional reconstruction were used to assess the radiologic healing status; a histologic analysis was performed to evaluate the pathologic process and relationship between osteogenesis and type H microvessels. Results-Well-remodeled trabecular meshworks were found in the low-intensity pulsed US treatment group compared to the control group. Micro-computed tomography and 3-dimensional reconstruction revealed more and thicker trabeculae after low-intensity pulsed US treatment. Abundant chondrocytes, a newly formed bone marrow cavity, trabeculae, and microvessels were formed at the fracture sites. More osterix-positive osteoblasts were circling the newly formed bone meshwork and were situated at the interface of chondrocytes in the low-intensity pulsed US treatment group. Type H microvessels were spreading around the newly formed trabecula, bone marrow cavity, osteoblasts, and interface of chondrocytes, with a larger mean vascular density in the low-intensity pulsed US group. Conclusions-Low-intensity pulsed US could accelerate traumatic vertebral fracture healing by temporally and spatially increasing chondrogenesis and osteoblast-induced osteogenesis coupled with angiogenesis of type H microvessels in a rat model of traumatic vertebral fracture.
引用
收藏
页码:1733 / 1742
页数:10
相关论文
共 50 条