In vitro ovine articular chondrocyte proliferation: experiments and modelling

被引：9

作者：

Mancuso, L. ^{[2
]}

Liuzzo, M. I. ^{[2
]}

Fadda, S. ^{[2
]}

Pisu, M. ^{[1
]}

Cincotti, A. ^{[2
]}

Arras, M. ^{[3
]}

La Nasa, G. ^{[3
]}

Concas, A. ^{[1
]}

Cao, G. ^{[1
,2
]}

机构：

[1] CRS4 Ctr Adv Studies Res & Dev Sardinia, I-09010 Cagliari, Italy

[2] Univ Cagliari, Dipartimento Ingn Chim & Mat, I-09123 Cagliari, Italy

[3] Ctr Trapianti di Midollo Osseo, Serv Diagnost Citofluorimetr & Trattamento Cellul, Cagliari, Italy

来源：

CELL PROLIFERATION | 2010年 / 43卷 / 03期

关键词：

POPULATION-BALANCE MODEL; ENGINEERED CARTILAGE GROWTH; SULFATED GLYCOSAMINOGLYCANS; STRUCTURED MODELS; TUMOR-GROWTH; CELL; PHENOTYPE;

D O I：

10.1111/j.1365-2184.2010.00676.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study focuses on analysis of in vitro cultures of chondrocytes from ovine articular cartilage. Isolated cells were seeded in Petri dishes, then expanded to confluence and phenotypically characterized by flow cytometry. The sigmoidal temporal profile of total counts was obtained by classic haemocytometry and corresponding cell size distributions were measured electronically using a Coulter Counter. A mathematical model recently proposed (1) was adopted for quantitative interpretation of these experimental data. The model is based on a 1-D (that is, mass-structured), single-staged population balance approach capable of taking into account contact inhibition at confluence. The model's parameters were determined by fitting measured total cell counts and size distributions. Model reliability was verified by predicting cell proliferation counts and corresponding size distributions at culture times longer than those used when tuning the model's parameters. It was found that adoption of cell mass as the intrinsic characteristic of a growing chondrocyte population enables sigmoidal temporal profiles of total counts in the Petri dish, as well as cell size distributions at 'balanced growth', to be adequately predicted.

引用

页码：310 / 320

页数：11

共 38 条

[1] Instantaneous evaluation of mammalian cell culture growth rates through analysis of the mitotic index [J].