Extracellular heat shock protein 60 (Hsp60) levels in children with septic shock

被引:22
作者
Wheeler, D. S.
Lahni, P.
Odoms, K.
Jacobs, B. R.
Carcillo, J. A.
Doughty, L. A.
Wong, H. R.
机构
[1] Univ Cincinnati, Coll Med, Dept Pediat, Cincinnati Childrens Hosp Med Ctr,Div Crit Care M, Cincinnati, OH 45229 USA
[2] Cincinnati Childrens Res Fdn, Kindervelt Lab Crit Care Med, Cincinnati, OH USA
[3] Univ Pittsburgh, Med Ctr, Dept Crit Care Med, Pittsburgh, PA 15260 USA
关键词
danger signal; heat shock proteins; Hsp60; sepsis; pediatrics; children; septic shock; SIRS; extra cellular;
D O I
10.1007/s00011-007-6108-4
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Objective and design: Recent data suggest that extracellular Hsp60 modulates the host innate immune response. We analyzed plasma Hsp60 levels in children admitted to a level III tertiary care PICU with septic shock. Materials and subjects: Blood samples were obtained from children meeting criteria for septic shock (n = 63), critically ill children without septic shock (n = 10), and healthy control s (n = 24). Treatment: Not applicable. Methods: Hsp60 levels were measured in the plasma using a commercially available ELISA. Differences between groups were analyzed with a Kruskal-Wallis one way ANOVA due to the non-parametric nature of the data. A p value <= 0.05 was considered significant. Results: Extracellular Hsp60 levels were significantly higher in children with septic shock (median, 16.7ng/mL) compared to both critically ill children without septic shock (median, 0ng/mL) and healthy controls (median, 0ng/mL, P < 0.001). Conclusions: Extracellular Hsp60 levels are significantly elevated in children with septic shock compared with both healthy controls and critically ill children without sepsis. Extracellular Hsp60 may play a role in the pathogenesis of sepsis in children.
引用
收藏
页码:216 / 219
页数:4
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