Primer3_masker: integrating masking of template sequence with primer design software

被引：337

作者：

Koressaar, Triinu ^{[1
]}

Lepamets, Maarja ^{[1
]}

Kaplinski, Lauris ^{[1
]}

Raime, Kairi ^{[1
]}

Andreson, Reidar ^{[1
]}

Remm, Maido ^{[1
]}

机构：

[1] Univ Tartu, Inst Mol & Cell Biol, Dept Bioinformat, Tartu, Estonia

来源：

BIOINFORMATICS | 2018年 / 34卷 / 11期

关键词：

GENOMES; SNPS;

D O I：

10.1093/bioinformatics/bty036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Summary: Designing PCR primers for amplifying regions of eukaryotic genomes is a complicated task because the genomes contain a large number of repeat sequences and other regions unsuitable for amplification by PCR. We have developed a novel k-mer based masking method that uses a statistical model to detect and mask failure-prone regions on the DNA template prior to primer design. We implemented the software as a standalone software primer3_masker and integrated it into the primer design program Primer3.

引用

页码：1937 / 1938

页数：2

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