Optical imaging of metabolic dynamics in animals

被引：193

作者：

Shi, Lingyan ^{[1
]}

Zheng, Chaogu ^{[2
]}

Shen, Yihui ^{[1
]}

Chen, Zhixing ^{[1
]}

Silveira, Edilson S. ^{[1
]}

Zhang, Luyuan ^{[1
]}

Wei, Mian ^{[1
]}

Liu, Chang ^{[1
]}

de Sena-Tomas, Carmen ^{[3
]}

Targoff, Kimara ^{[3
]}

Min, Wei ^{[1
,4
]}

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

[2] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

[3] Columbia Univ, Dept Pediat, New York, NY 10027 USA

[4] Columbia Univ, Kavli Inst Brain Sci, New York, NY 10027 USA

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

关键词：

RAMAN-SCATTERING MICROSCOPY; IN-VIVO; DEUTERIUM-OXIDE; GENE-EXPRESSION; CELL-DIVISION; WATER; LIPOGENESIS; RATES; ACID;

D O I：

10.1038/s41467-018-05401-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Direct visualization of metabolic dynamics in living animals with high spatial and temporal resolution is essential to understanding many biological processes. Here we introduce a platform that combines deuterium oxide (D2O) probing with stimulated Raman scattering (DO-SRS) microscopy to image in situ metabolic activities. Enzymatic incorporation of D2O-derived deuterium into macromolecules generates carbon-deuterium (C-D) bonds, which track biosynthesis in tissues and can be imaged by SRS in situ. Within the broad vibrational spectra of C-D bonds, we discover lipid-, protein-, and DNA-specific Raman shifts and develop spectral unmixing methods to obtain C-D signals with macromolecular selectivity. DO-SRS microscopy enables us to probe de novo lipogenesis in animals, image protein biosynthesis without tissue bias, and simultaneously visualize lipid and protein metabolism and reveal their different dynamics. DO-SRS microscopy, being noninvasive, universally applicable, and cost-effective, can be adapted to a broad range of biological systems to study development, tissue homeostasis, aging, and tumor heterogeneity.

引用

页数：17

共 66 条

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