Comparison of RNA extraction methods applied to gene cloning of the taxol-producing fungi

被引：0

作者：

Ye, Wei ^{[1
,2
,3
]}

Liu, Lu ^{[1
,2
]}

Zheng, Wenlong ^{[1
,2
,3
]}

Lin, Juan ^{[1
,2
]}

Yin, Jingyuan ^{[3
]}

机构：

[1] Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

[2] Fudan Univ, Sch Life Sci, Inst Plant Biol, Shanghai 200433, Peoples R China

[3] Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China

来源：

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2009年 / 3卷 / 10期

关键词：

Taxol; taxol-producing endophytic fungi; RNA extracting; RT-PCR; ENDOPHYTIC FUNGUS; TRANSFORMATION; TAXUS;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The qualities of RNA are an important foundation for molecular biology research. In this study, five different methods were selected for extracting RNA from the taxol-producing endophytic fungi. Yield and quality of isolated RNA were assessed by routine test method. The results showed that the Trizol method perform was better than others method in the yield, but the LiCl method perform better than others methods in quality using A(260/280) and A(260/230) ratios and agarose electrophoresis results. Further, using the RNA of extracting from fungi by LiCl method, 10-deacetylbaccatin III-10-O-acetyltransferase (DBAT) gene partial sequence were successful amplified using RT-PCR method. This study laid a foundation for further gene clone and construction of gene library.

引用

页码：632 / 636

页数：5

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