Determination of polymyxin E1 in rat plasma by high-performance liquid chromatography

被引：26

作者：

Gmur, DJ ^{[1
]}

Bredl, CR ^{[1
]}

Steele, SJ ^{[1
]}

Cai, SP ^{[1
]}

VanDevanter, DR ^{[1
]}

Nardella, PA ^{[1
]}

机构：

[1] Chiron Corp, Seattle, WA 98119 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 789卷 / 02期

关键词：

polymyxin E-1;

D O I：

10.1016/S1570-0232(03)00162-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A precise and accurate HPLC assay for polymyxin E-1 in rat and dog plasma has been validated. Samples and standards are extracted from plasma with a 96-well C-8 extraction disk plate. Sample extracts are derivatized with dansyl chloride, and polymyxin E-1 derivative is quantitated on a C-8 column by HPLC with fluorescence detection. The assay is linear in the range of 0.050-5.00 mug/ml for polyrnyxim E-1. The precision and accuracy of polymyxin E-1 plasma assay was well within the recommended limits set in the FDA Guidance for Bioanalytical Method Validation. Polymyxin E-1 stability in rat and dog plasma for 24 h at room temperature and through three freeze-thaw cycles was demonstrated. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：365 / 372

页数：8