Effects of zinc deficiency and pneumococcal surface protein A immunization on zinc status and the risk of severe infection in mice

被引:32
作者
Strand, TA [1 ]
Hollingshead, SK
Julshamn, K
Briles, DE
Blomberg, B
Sommerfelt, H
机构
[1] Univ Bergen, Ctr Int Hlth, N-5021 Bergen, Norway
[2] Univ Bergen, Gade Inst, Dept Microbiol & Immunol, N-5021 Bergen, Norway
[3] Inst Nutr, Directorate Fisheries, N-5804 Bergen, Norway
[4] Univ Alabama Birmingham, Dept Microbiol, Birmingham, AL 35294 USA
关键词
D O I
10.1128/IAI.71.4.2009-2013.2003
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Streptococcus pneumoniae is a major cause of illness and death in children in developing countries. In these children, zinc deficiency is associated with an increased risk of acute respiratory tract infections, which can be reduced by daily zinc administration. Severe infections decrease zinc levels in plasma and may thereby move individuals with preexisting low zinc stores into a vicious cycle of infection and unavailable zinc. Pneumococcal surface protein A (PspA) has emerged as a promising vaccine candidate, and immunization with this antigen protects animals from pneumococcal infection. In an animal experiment, we measured the effect of zinc depletion on the immune response to parenterally administrated PspA and assessed the effect of this PspA vaccination and zinc depletion on the severity of pneumococcal infection and on zinc status. Mice were kept on different diets for 5 weeks, immunized twice 14 days apart, and challenged intranasally with S. pneumoniae. Mice on the zinc-deficient diet showed substantially reduced immune responses to PspA, more extensive pneumococcal colonization in the nasal mucosa, more severe infections, and an increased risk of death. PspA immunization reduced the risk of severe disease, and the reduction in severity was reflected in substantially reduced zinc depletion from bones.
引用
收藏
页码:2009 / 2013
页数:5
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