Quantitative immunocapture PCR assay for detection of Campylobacter jejuni in foods

被引：32

作者：

Waller, DF ^{[1
]}

Ogata, SA ^{[1
]}

机构：

[1] Hawaii Biotechnol Grp Inc, Aiea, HI 96701 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 09期

关键词：

D O I：

10.1128/AEM.66.9.4115-4118.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The rapid detection of food-borne bacterial pathogens as part of a quality control program is necessary for the maintenance of a safe food supply. In this report, we present our findings for an immunocapture PCR method for the detection of Campylobacter jejuni in foods. The method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 8 h. Assay results are quantitative, and one cell in a milliliter sample can be detected. Application of the method to spiked milk samples and chicken skin washes did not affect the sensitivity of the assay.

引用

收藏

页码：4115 / 4118

页数：4

相关论文

共 22 条

[1] Campylobacter jejuni -: An emerging foodborne pathogen [J].

Altekruse, SF ;

Stern, NJ ;

Fields, PI ;

Swerdlow, DL .

EMERGING INFECTIOUS DISEASES, 1999, 5 (01) :28-35

[2] DISTRIBUTION AND NUMBERS OF CAMPYLOBACTER-IN NEWLY SLAUGHTERED BROILER-CHICKENS AND HENS [J].

BERNDTSON, E ;

TIVEMO, M ;

ENGVALL, A .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1992, 15 (1-2) :45-50

[3]

BLACK RE, 1992, CAMPLYLOBACTER JEJUNI, P207

[4] The magnetic immuno-polymerase chain reaction assay for the detection of Campylobacter in milk and poultry [J].

Docherty, L ;

Adams, MR ;

Patel, P ;

McFadden, J .

LETTERS IN APPLIED MICROBIOLOGY, 1996, 22 (04) :288-292

[5] RAPID AND SENSITIVE DETECTION OF CAMPYLOBACTER SPP IN CHICKEN PRODUCTS BY USING THE POLYMERASE CHAIN-REACTION [J].

GIESENDORF, BAJ ;

QUINT, WGV ;

HENKENS, MHC ;

STEGEMAN, H ;

HUF, FA ;

NIESTERS, HGM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (12) :3804-3808

[6]

GOLDENBERGER D, 1995, PCR METH APPL, V4, P368

[7] CAMPYLOBACTERS ASSOCIATED WITH HUMAN DIARRHEAL DISEASE [J].

GRIFFITHS, PL ;

PARK, RWA .

JOURNAL OF APPLIED BACTERIOLOGY, 1990, 69 (03) :281-301

[8] DETECTION OF THE COCCOID FORM OF CAMPYLOBACTER-JEJUNI IN CHICKEN PRODUCTS WITH THE USE OF THE POLYMERASE CHAIN-REACTION [J].

HAZELEGER, W ;

ARKESTEIJN, C ;

TOOROPBOUMA, A ;

BEUMER, R .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1994, 24 (1-2) :273-281

[9] DETECTION OF SHIGELLA-DYSENTERIAE TYPE-1 AND SHIGELLA-FLEXNERI IN FECES BY IMMUNOMAGNETIC ISOLATION AND POLYMERASE CHAIN-REACTION [J].

ISLAM, D ;

LINDBERG, AA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) :2801-2806

[10]

LUK JMC, 1994, BIOTECHNIQUES, V17, P1038