Cryo-EM structure of the human IgM B cell receptor

被引：49

作者：

Su, Qiang ^{[1
,2
]}

Chen, Mengying ^{[3
]}

Shi, Yan ^{[1
,2
,4
]}

Zhang, Xiaofeng ^{[1
,2
]}

Huang, Gaoxingyu ^{[1
,2
]}

Huang, Bangdong ^{[1
,2
]}

Liu, Dongwei ^{[4
]}

Liu, Zhangsuo ^{[4
]}

Shi, Yigong ^{[1
,2
,3
]}

机构：

[1] Westlake Univ, Res Ctr Ind Future, Sch Life Sci, Inst Biol,Westlake Inst Adv Study,Key Lab Struct, Hangzhou 310024, Zhejiang, Peoples R China

[2] Westlake Lab Life Sci & Biomed, Hangzhou 310024, Zhejiang, Peoples R China

[3] Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Sch Life Sci,Sch Med, Beijing 100084, Peoples R China

[4] Zhengzhou Univ, Affiliated Hosp 1, Henan Prov Res Ctr Kidney Dis, Dept Integrated Tradit & Western Nephrol, Zhengzhou 450052, Peoples R China

来源：

SCIENCE | 2022年 / 377卷 / 6608期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

ANTIGEN RECEPTOR; CRYSTAL-STRUCTURE; ORGANIZATION; INITIATION; ALPHA; BETA;

D O I：

10.1126/science.abo3923

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The B cell receptor (BCR) initiates immune responses through antigen recognition. We report a 3.3-angstrom cryo-electron microscopy structure of human immunoglobulin M (IgM)-BCR in the resting state. IgM-BCR comprises two heavy chains, two light chains, and the Ig alpha/Ig beta heterodimer. The ectodomains of the heavy chains closely stack against those of Ig alpha/Ig beta, with one heavy chain locked between Ig alpha and Ig beta in the juxtamembrane region. Extracellular interactions may determine isotype specificity of the BCR. The transmembrane helices of IgM-BCR form a four-helix bundle that appears to be conserved among all BCR isotypes. This structure contains 14 glycosylation sites on the IgM-BCR ectodomains and reveals three potential surface binding sites. Our work reveals the organizational principles of the BCR and may facilitate the design of antibody-based therapeutics.

引用

页码：875 / +

页数：6

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