The Etest Performed Directly on Blood Culture Bottles Is a Reliable Tool for Detection of Fluconazole-Resistant Candida albicans Isolates

被引：0
作者：
Escribano, Pilar [1 ,2 ]
Judith Marcos-Zambrano, Laura [1 ,2 ]
Gomez, Ana [1 ,2 ]
Sanchez, Carlos [1 ,2 ,3 ]
Carmen Martinez-Jimenez, M. [1 ,2 ]
Bouza, Emilio [1 ,2 ,3 ,4 ]
Guinea, Jesus [1 ,2 ,3 ,4 ]
机构：
[1] Univ Complutense Madrid, Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain
[2] Hosp Gregorio Maranon, Inst Invest Sanitaria, Madrid, Spain
[3] CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain
[4] Univ Complutense Madrid, Sch Med, Med Dept, Madrid, Spain
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2017年 / 61卷 / 07期
关键词：
Candida albicans; fluconazole; resistance; Etest; MINIMUM INHIBITORY CONCENTRATIONS; END-POINT PHENOTYPE; ANTIFUNGAL SUSCEPTIBILITY; AZOLE RESISTANCE; IN-VITRO; ERG11; MUTATIONS; RISK-FACTOR; MORTALITY; FUNGEMIA; IDENTIFICATION;
D O I：
10.1128/AAC.00400-17
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We assessed the ability of the Etest performed directly on positive blood cultures (ETDIR) to detect fluconazole susceptibility in 6 fluconazole-resistant and 12 fluconazole-susceptible Candida albicans isolates, according to CLSI M27-A3 and EUCAST EDef 7.2 procedures. Categorical agreement between ETDIR and broth microdilution was 100% when the trays were incubated at 25 degrees C and trailing effect was ruled out. ETDIR is a reliable procedure when screening for the presence of fluconazole resistance in C. albicans.
引用
收藏
页数：7
相关论文
共 47 条
  • [1] Trailing end-point phenotype of Candida spp. in antifungal susceptibility testing to fluconazole is eliminated by altering incubation temperature
    Agrawal, D.
    Patterson, T. F.
    Rinaldi, M. G.
    Revankar, S. G.
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2007, 56 (07) : 1003 - 1004
  • [2] Influence of different susceptibility testing methods and media on determination of the relevant fluconazole minimum inhibitory concentrations for heavy trailing Candida isolates with low-high phenotype
    Alp, Sehnaz
    Sancak, Banu
    Hascelik, Gulsen
    Arikan, Sevtap
    [J]. MYCOSES, 2010, 53 (06) : 475 - 480
  • [3] [Anonymous], 2008, Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts
  • [4] Third Informational Supplement. CLSI document M27-S3, P28
  • [5] EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST)
    Arendrup, Maiken C.
    Cuenca-Estrella, Manuel
    Lass-Floerl, Cornelia
    Hope, William
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (07) : E246 - E247
  • [6] Avolio M, 2009, NEW MICROBIOL, V32, P179
  • [7] A Combination Fluorescence Assay Demonstrates Increased Efflux Pump Activity as a Resistance Mechanism in Azole-Resistant Vaginal Candida albicans Isolates
    Bhattacharya, Somanon
    Sobel, Jack D.
    White, Theodore C.
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2016, 60 (10) : 5858 - 5866
  • [8] Antifungal Susceptibility of 205 Candida spp. Isolated Primarily during Invasive Candidiasis and Comparison of the Vitek 2 System with the CLSI Broth Microdilution and Etest Methods
    Bourgeois, N.
    Dehandschoewercker, L.
    Bertout, S.
    Bousquet, P. -J.
    Rispail, P.
    Lachaud, L.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) : 154 - 161
  • [9] Evaluation of etest for direct antifungal susceptibility testing of yeasts in positive blood cultures
    Chang, HC
    Chang, JJ
    Chan, SH
    Huang, AH
    Wu, TL
    Lin, MC
    Chang, TC
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (04) : 1328 - 1333
  • [10] Overexpression of CDR1 and CDR2 Genes Plays an Important Role in Fluconazole Resistance in Candida albicans with G487T and T916C Mutations
    Chen, L. M.
    Xu, Y. H.
    Zhou, C. L.
    Zhao, J.
    Li, C. Y.
    Wang, R.
    [J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 2010, 38 (02) : 536 - 545
12345