Optical tweezers for measuring red blood cell elasticity: application to the study of drug response in sickle cell disease

被引:196
作者
Brandao, MM
Fontes, A
Barjas-Castro, ML
Barbosa, LC
Costa, FF
Cesar, CL
Saad, STO
机构
[1] State Univ Campinas, UNICAMP, Dept Internal Med, Sao Paulo, Brazil
[2] State Univ Campinas, UNICAMP, Haemoctr, Sao Paulo, Brazil
[3] State Univ Campinas, UNICAMP, Phys Inst Gleb Wataghin, Sao Paulo, Brazil
关键词
optical tweezers; sickle cell; hydroxyurea; red blood cell elasticity; deformability; DEFORMABILITY; ERYTHROCYTES; HEMOGLOBIN; HYDROXYUREA;
D O I
10.1034/j.1600-0609.2003.00027.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The deformability of erythrocytes is a critical determinant of blood flow in microcirculation. By capturing red blood cells (RBC) with optical tweezers and dragging them through a viscous fluid we were able to measure their overall elasticity. We measured, and compared, the RBC deformability of 15 homozygous patients (HbSS) including five patients taking hydroxyurea (HU) for at least 6 months (HbSS/HU), 10 subjects with sickle cell trait (HbAS) and 35 normal controls. Our results showed that the RBC deformability was significantly lower in haemoglobin S (HbS) subjects (HbSS and HbAS), except for HbSS/HU cells, whose deformability was similar to the normal controls. Our data showed that the laser optical tweezers technique is able to detect differences in HbS RBC from subjects taking HU, and to differentiate RBC from normal controls and HbAS, indicating that this is a very sensitive method and can be applied for detection of drug-response in sickle cell disease.
引用
收藏
页码:207 / 211
页数:5
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