Determination of Ochratoxin A in Wine by High-Performance Thin-Layer Chromatography using Charged Coupled Device

被引：27

作者：

Welke, Juliane E. ^{[1
]}

Hoeltz, Michele ^{[1
]}

Dottori, Horacio A. ^{[2
]}

Noll, Isa B. ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Inst Ciencia & Tecnol Alimentos, BR-91570901 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Inst Fis, BR-91570901 Porto Alegre, RS, Brazil

来源：

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY | 2010年 / 21卷 / 03期

关键词：

ochratoxin A; wine; charge-coupled device imaging; high-performance thin-layer chromatography; LIQUID-CHROMATOGRAPHY; RENAL TUMORS; APPLE JUICE; QUANTIFICATION; ASPERGILLUS; CLEANUP; PATULIN; RATS;

D O I：

10.1590/S0103-50532010000300007

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method was developed and validated in-house for determination of ochratoxin A (OTA) in wine using charge coupled device (CCD) to acquire the fluorescence images of mycotoxin from high-performance thin-layer chromatography (HPTLC) under UV lamp. The method showed a mean recovery of 90.4%. The quantification and detection limist were 0.1 mu g L-1 and 0.016 mu g L-1 per spot, respectively. The results of validation confirmed the efficiency of the method, which is sensitive enough to be used to quantify OTA in wine. The occurrence of OTA in Brazilian wines was evaluated. OTA was found in one sample at a level of 4.5 mu g L-1, which is higher than the limit of 2 mu g L-1 considered acceptable by the Scientific Commission of the European Communities. This study demonstrated the applicability of HPTLC using CCD as a tool to determine OTA in wine.

引用

页码：441 / 446

页数：6

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