ATPase family AAA domain-containing 3A is a novel anti-apoptotic factor in lung adenocarcinoma cells

被引：76

作者：

Fang, Hsin-Yuan ^{[1
,2
]}

Chang, Chia-Ling ^{[3
]}

Hsu, Shu-Han ^{[3
]}

Huang, Chih-Yang ^{[4
]}

Chiang, Shu-Fen ^{[4
]}

Chiou, Shiow-Her ^{[4
]}

Huang, Chun-Hua ^{[3
]}

Hsiao, Yi-Ting ^{[3
]}

Lin, Tze-Yi ^{[5
]}

Chiang, I-Ping ^{[5
]}

Hsu, Wen-Hu ^{[6
]}

Sugano, Sumio ^{[7
]}

Chen, Chih-Yi ^{[2
]}

Lin, Ching-Yuang ^{[2
]}

Ko, Wen-Je ^{[1
]}

Chow, Kuan-Chih ^{[3
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Clin Med, Taipei 10764, Taiwan

[2] China Med Univ, Dept Surg, China Med Univ Hosp, Taichung, Taiwan

[3] Natl Chung Hsing Univ, Grad Inst Biomed Sci, Taichung 40227, Taiwan

[4] Natl Chung Hsing Univ, Grad Inst Microbiol & Publ Hlth, Taichung 40227, Taiwan

[5] China Med Univ Hosp, Dept Pathol, Taichung, Taiwan

[6] Taipei Vet Gen Hosp, Div Thorac Surg, Dept Surg, Taipei, Taiwan

[7] Univ Tokyo, Lab Funct Genom, Dept Med Genome Sci, Grad Sch Frontier Sci, Tokyo, Japan

来源：

JOURNAL OF CELL SCIENCE | 2010年 / 123卷 / 07期

关键词：

ATAD3A; Lung adenocarcinoma; Mitochondrial fragmentation; Cisplatin sensitivity; PROTEIN-KINASE-C; HEPATOCYTE GROWTH-FACTOR; CANCER CELLS; MITOCHONDRIAL FRAGMENTATION; EXPRESSION; IDENTIFICATION; MITOFUSIN-2; ACTIVATION; MEMBRANE; HYPOXIA;

D O I：

10.1242/jcs.062034

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

AAA domain-containing 3A (ATAD3A) is a member of the AAA-ATPase family. Three forms of ATAD3 have been identified: ATAD3A, ATAD3B and ATAD3C. In this study, we examined the type and expression of ATAD3 in lung adenocarcinoma (LADC). Expression of ATAD3A was detected by reverse transcription-polymerase chain reaction, immunoblotting, immunohistochemistry and confocal immunofluorescent microscopy. Our results show that ATAD3A is the major form expressed in LADC. Silencing of ATAD3A expression increased mitochondrial fragmentation and cisplatin sensitivity. Serum deprivation increased ATAD3A expression and drug resistance. These results suggest that ATAD3A could be an anti-apoptotic marker in LADC.

引用

页码：1171 / 1180

页数：10

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