The essential oil (obtained by hydrodistillation) and hexane extracts (obtained by Soxhlet apparatus) of Chaerophyllum macropodum (Boiss.) from two different area were analyzed by GC and GC-MS. The essential oil of C. macropodum from Givi (sample A) and Neor (sample B) localities were characterized by a dominant amount of trans-beta-ocimene (22.8% and 21.2%), cis-beta- ocimene (10.0% and 8.5%) and (+) spathulenol (4.4% and 6.0%), respectively. p-cymene (10.3%), gamma-terpinene (9.2%), trans-beta-farnesene (8.2 %) and fenchyl acetate (6.5%) among the 27 components of the total oil in Givi sample were detected. Caryophyllene oxide (9.0%), alpha-pinene (7.1%), beta- caryophyllene (6.6%), myristicin (5.1%) and sabinene (5.0%) were predominated in the oil obtained from Neor sample. In the hexane extracts of sample A and B the following compounds were detected, respectively: linoleic acid (23.2% and 29.2%), 6-octadecenoic acid (20.2% and 6.8%), 9, 12-octadecadienoic acid (8.8% and 9.9%) and E-beta-farnesene (4.6% and 8.7%). Carotol (16.9%) and (-)-beta- acoradiene (5.1%) were the major compound in the organic extract of sample A and germacrene- D (8.8%) and acorenone-B (12.6%) were the major ones in sample B. The constituents of the both oils were different, although the most abundant components are identical in essential oil from A (Givi sample) (99.3%). The antimicrobial activity of essential oils and hexane extracts from C. macropodum were studied according to the agar diffusion cup method. The essential oils had a good effect but the organic extracts had a moderate effect on the some Gram-positive and Gram-negative bacteria.
