Epstein-Barr viral load as a marker of lymphoma in AIDS patients

被引:54
作者
Fan, HX
Kim, SC
Chima, CO
Israel, BF
Lawless, KM
Eagan, PA
Elmore, S
Moore, DT
Schichman, SA
Swinnen, LJ
Gulley, ML
机构
[1] Univ N Carolina, Dept Pathol, Chapel Hill, NC 27599 USA
[2] Lineberger Comprehens Canc Ctr, Chapel Hill, NC USA
[3] Univ Texas, Hlth Sci Ctr, San Antonio, TX USA
[4] Univ Arkansas Med Sci, Little Rock, AR 72205 USA
[5] Cent Arkansas Vet Healthcare Syst, Little Rock, AR USA
[6] Johns Hopkins Univ, Baltimore, MD USA
关键词
Epstein-Barr virus; viral load; AIDS; lymphoma; tumor marker;
D O I
10.1002/jmv.20238
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Epstein-Barr virus (EBV) is implicated in the pathogenesis of acquired immunodeficiency syndrome (AIDS) lymphoma, and viral DNA is present within the malignant cells in about half of affected patients. We examined the extent to which EBV viral load is elevated in the plasma of AIDS lymphoma patients compared to AIDS patients with opportunistic infections. Sixty-one AIDS patients were studied including 35 with lymphoma (24 non-Hodgkin, six Hodgkin, and five brain lymphoma) and 26 with various opportunistic infections. In situ hybridization revealed EBV encoded RNA (EBER) expression in the malignant cells of 17/28 AIDS lymphomas (61%). In 232 serial plasma samples from 35 lymphoma patients and in 128 samples from AIDS controls, EBV viral load was assayed by quantitative-polymerase chain reaction (Q-PCR) using a TaqMan probe targeting the BamH1W sequence. EBV was detected in plasma from all 17 EBER-positive AIDS lymphoma patients, with viral loads ranging from 34 to 1,500,000 copies per ml (median 3,210). Viral load usually fell rapidly upon initiation of lymphoma therapy and remained undetectable except in two patients with persistent tumor. In 11 AIDS patients, whose lymphoma lacked EBER expression, and in 26 control patients without lymphoma, levels of EBV in plasma were usually low or undetectable (range 0-1,995 and 0-2,409, median 0 and 0, respectively). There was no association between EBV viral load and human immunodeficiency virus (HIV) load or CD4 count. In conclusion, EBV viral load shows promise as a tool to assist in diagnosis and management of EBV-related lymphoma patients. (C) 2005Wiley-Liss, Inc.
引用
收藏
页码:59 / 69
页数:11
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