A SiO2-coated nanoporous alumina membrane for stable label-free waveguide biosensing

被引:9
作者
Fan, Yong [1 ,2 ]
Ding, Yu [1 ,2 ]
Zhang, Yafei [1 ]
Ma, Hui [1 ,2 ]
He, Yonghong [1 ]
Sun, Shuqing [1 ]
机构
[1] Tsinghua Univ, Grad Sch Shenzhen, Shenzhen Key Lab Minimal Invas Med Technol, Shenzhen 518055, Peoples R China
[2] Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China
来源
RSC ADVANCES | 2014年 / 4卷 / 108期
关键词
OPTICAL-PROPERTIES; THIN-FILMS; DEPOSITION; TIO2; TEMPLATES; SURFACES; GLUCOSE; ARRAYS; SENSOR; FIELD;
D O I
10.1039/c4ra08839e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Nanoporous anodic alumina (PAA) has been widely employed in many areas due to its well-ordered and self-organized pore structures. In the present study, we demonstrated two uses of a SiO2 coating: to attach a free-standing PAA membrane to a hydroxylated gold layer for use as a planar optical waveguide sensor and to create a protective layer against etching in aqueous solution by a surface sol-gel (SSG) method. Scanning electron microscopy (SEM) indicates that the as-formed SiO2 layer covers both the inner and outer surfaces of the PAA membrane. Optical waveguide spectroscopy (OWS) measurements show that the apparent waveguide modes were typical for a PAA/Au film with "SiO2 glue", while only the typical surface plasma resonance curve was observed before the SSG process was performed. Additionally, the SiO2-coated PAA/Au film after five SSG cycles displays greater aqueous stability in the range of pH 2.4 to 7.3 in comparison to the primary PAA membrane, indicating that the SiO2 layer was continuous. The feasibility of label-free optical biosensing was demonstrated by two immunosensor experiments that monitored the adsorption of either bovine serum albumin (BSA) or streptavidin in real time. The application of the SSG method to a thicker PAA film (>2.5 mu m) with an organized pore structure for OWS biosensing was demonstrated, and improved sensitivity in good agreement with simulation results was achieved.
引用
收藏
页码:62987 / 62995
页数:9
相关论文
共 45 条
[1]   Micro/nanoporous polymer chips as templates for highly sensitive SERS sensors [J].
Akil-Jradi, Suzanna ;
Jradi, Safi ;
Plain, Jerome ;
Adam, Pierre-Michel ;
Bijeon, Jean-Louis ;
Royer, Pascal ;
Bachelot, Renaud .
RSC ADVANCES, 2012, 2 (20) :7837-7842
[2]   OPTICAL-PROPERTIES OF THIN-FILMS [J].
ASPNES, DE .
THIN SOLID FILMS, 1982, 89 (03) :249-262
[3]   AN INTEGRATED SILICON THERMOPILE AS BIOSENSOR FOR THE THERMAL MONITORING OF GLUCOSE, UREA AND PENICILLIN [J].
BATAILLARD, P ;
STEFFGEN, E ;
HAEMMERLI, S ;
MANZ, A ;
WIDMER, HM .
BIOSENSORS & BIOELECTRONICS, 1993, 8 (02) :89-98
[4]  
Brinker C. J., 2013, SOL GEL SCI PHYS CHE, DOI [DOI 10.1016/B978-0-08-057103-4.50001-5, 10.1016/C2009-0-22386-5]
[5]   Controlled thermal decomposition of aromatic polyethers to attain nanoporous carbon materials with enhanced gas storage [J].
Cabasso, Israel ;
Li, Suoding ;
Wang, Xinwei ;
Yuan, Youxin .
RSC ADVANCES, 2012, 2 (10) :4079-4091
[6]   An investigation of antibody immobilization methods employing organosilanes on planar ZnO surfaces for biosensor applications [J].
Corso, Christopher D. ;
Dickherber, Anthony ;
Hunt, William D. .
BIOSENSORS & BIOELECTRONICS, 2008, 24 (04) :805-811
[7]   Optical waveguide sensor based on silica nanotube arrays for label-free biosensing [J].
Fan, Yong ;
Ding, Yu ;
Ma, Hui ;
Teramae, Norio ;
Sun, Shuqing ;
He, Yonghong .
BIOSENSORS & BIOELECTRONICS, 2015, 67 :230-236
[8]   Highly sensitive real-time detection of DNA hybridization by using nanoporous waveguide fluorescence spectroscopy [J].
Fan, Yong ;
Hotta, Kazuhiro ;
Yamaguchi, Akira ;
Ding, Yu ;
He, Yonghong ;
Teramae, Norio ;
Sun, Shuqing ;
Ma, Hui .
APPLIED PHYSICS LETTERS, 2014, 105 (03)
[9]   Enhanced fluorescence in a nanoporous waveguide and its quantitative analysis [J].
Fan, Yong ;
Hotta, Kazuhiro ;
Yamaguchi, Akira ;
Teramae, Norio .
OPTICS EXPRESS, 2012, 20 (12) :12850-12859
[10]   TEMPLATE SYNTHESIS OF INFRARED-TRANSPARENT METAL MICROCYLINDERS - COMPARISON OF OPTICAL-PROPERTIES WITH THE PREDICTIONS OF EFFECTIVE MEDIUM THEORY [J].
FOSS, CA ;
TIERNEY, MJ ;
MARTIN, CR .
JOURNAL OF PHYSICAL CHEMISTRY, 1992, 96 (22) :9001-9007