Mechanisms of Yangjing Capsule in Leydig Cell Apoptosis and Testosterone Synthesis via Promoting StAR Expression

被引：13

作者：

Sun, Dalin ^{[1
]}

Dong, Weihang ^{[2
]}

Jin, Baofang ^{[1
]}

Chen, Guanghui ^{[3
]}

Cai, Bin ^{[1
]}

Deng, Weimin ^{[1
]}

Cui, Yugui ^{[4
]}

Jin, Yihan ^{[2
]}

机构：

[1] Southeast Univ, Zhongda Hosp, Sch Med, Androl Dept Integrat Med, Nanjing 210009, Jiangsu, Peoples R China

[2] Qinghai Univ, Med Coll, Xining 810001, Qinghai, Peoples R China

[3] Hebei Prov Hosp Tradit Chinese Med, Shijiazhuang 050011, Hebei, Peoples R China

[4] Nanjing Med Univ, Affiliated Hosp 1, Clin Ctr Reprod Med, State Key Lab Reprod Med, Nanjing 210029, Jiangsu, Peoples R China

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2018年 / 41卷 / 09期

关键词：

Yangjing Capsule (YC); Leydig cell; apoptosis; testosterone; steroidogenic acute regulatory protein (StAR); TRANSCRIPTION;

D O I：

10.1248/bpb.b18-00205

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The present study aims to investigate the roles of steroidogenic acute regulatory protein (StAR) in Yangjing Capsule (YC) induced anti-apoptotic effects on Leydig cells and the related mechanism. Leydig tumor cells (MLTC-1) were cultured and treated with YC, and immunofluorescence assay was performed to examine the expression of StAR; furthermore, luciferase reporter assay was conducted to evaluate the impact of YC on StAR promoter; next, MLTC-1 cells were treated with StAR small interfering RNA (siRNA), and flow cytometry was carried out to examine the effect of StAR siRNA on the apoptosis of the cells; furthermore, quantitative (q)RT-PCR and Western blot methods was used to determine the expression of StAR and apoptosis related molecules Bcl-2, Bax and Caspase-3 on both mRNA and protein levels in different groups; finally the secretion of testosterone in different groups was examined by radioimmunoassay. We observed that the YC can increase the expression of StAR in a dose-dependent manner, and YC can activate the promoter of StAR; moreover, transfection of StAR siRNA can block YC induced anti-apoptotic effects and increased production of testosterone. In conclusion, our results suggested that YC might suppress the apoptosis of MLTC-1 cells and enhance the production of testosterone through regulating the expression of StAR.

引用

页码：1401 / 1405

页数：5

共 22 条

[11] Apoptosome activation, an important molecular instigator in 6-mercaptopurine induced Leydig cell death [J].

Morgan, Jessica A. ;

Lynch, John ;

Panetta, John C. ;

Wang, Yao ;

Frase, Sharon ;

Bao, Ju ;

Zheng, Jie ;

Opferman, Joseph T. ;

Janke, Laura ;

Green, Daniel M. ;

Chemaitilly, Wassim ;

Schuetz, John D. .

SCIENTIFIC REPORTS, 2015, 5

[12] hCG-induced endoplasmic reticulum stress triggers apoptosis and reduces steroidogenic enzyme expression through activating transcription factor 6 in Leydig cells of the testis [J].

Park, Sun-Ji ;

Kim, Tae-Shin ;

Park, Choon-Keun ;

Lee, Sang-Hee ;

Kim, Jin-Man ;

Lee, Kyu-Sun ;

Lee, In-kyu ;

Park, Jeen-Woo ;

Lawson, Mark A. ;

Lee, Dong-Seok .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2013, 50 (02) :151-166

[13] Mono-2-Ethylhexyl Phthalate Stimulates Androgen Production but Suppresses Mitochondrial Function in Mouse Leydig Cells With Different Steroidogenic Potential [J].

Savchuk, Iuliia ;

Soder, Olle ;

Svechnikov, Konstantin .

TOXICOLOGICAL SCIENCES, 2015, 145 (01) :149-156

[14] Single-molecule fluorescence microscopy review: shedding new light on old problems [J].

Shashkova, Sviatlana ;

Leake, Mark C. .

BIOSCIENCE REPORTS, 2017, 37

[15] Parathyroid Hormone-Related Protein Promotes Rat Stem Leydig Cell Differentiation [J].

Song, Tiantian ;

Wang, Yiyan ;

Li, Huitao ;

Chen, Lanlan ;

Liu, Jianpeng ;

Chen, Xianwu ;

Li, Xiaojun ;

Li, Xiaoheng ;

Li, Linxi ;

Lian, Qingquan ;

Ge, Ren-Shan .

FRONTIERS IN PHYSIOLOGY, 2017, 8

[16] Elevated levels of the steroidogenic factor 1 are associated with over-expression of CYP19 in an oestrogen-producing testicular Leydig cell tumour [J].

Straume, Anne Hege ;

Lovas, Kristian ;

Miletic, Hrvoje ;

Gravdal, Karsten ;

Lonning, Per Eystein ;

Knappskog, Stian .

EUROPEAN JOURNAL OF ENDOCRINOLOGY, 2012, 166 (05) :941-949

[17] Effects of the Yangjing Capsule Extract on Steroidogenesis and Apoptosis in Mouse Leydig Cells [J].

Sun, Dalin ;

Cui, Yugui ;

Jin, Baofang ;

Zhang, Xindong ;

Yang, Xiaoyu ;

Gao, Chao .

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2012, 2012

[18]

Wang Z, 2015, EVID-BASED COMPL ALT, V2015

[19] Mullerian Inhibiting Substance Recruits ALK3 to Regulate Leydig Cell Differentiation [J].

Wu, Xiufeng ;

Zhang, Ningning ;

Lee, Mary M. .

ENDOCRINOLOGY, 2012, 153 (10) :4929-4937

[20] Protective effect of calretinin on testicular Leydig cells via the inhibition of apoptosis [J].

Xu, Wendan ;

Zhu, Qian ;

Zhang, Bei ;

Liu, Shan ;

Dai, Xiaonan ;

Gao, Chao ;

Gao, Li ;

Cui, Yugui .

AGING-US, 2017, 9 (04) :1269-1279

← 1 2 3 →