Mechanisms of Yangjing Capsule in Leydig Cell Apoptosis and Testosterone Synthesis via Promoting StAR Expression

被引：13

作者：

Sun, Dalin ^{[1
]}

Dong, Weihang ^{[2
]}

Jin, Baofang ^{[1
]}

Chen, Guanghui ^{[3
]}

Cai, Bin ^{[1
]}

Deng, Weimin ^{[1
]}

Cui, Yugui ^{[4
]}

Jin, Yihan ^{[2
]}

机构：

[1] Southeast Univ, Zhongda Hosp, Sch Med, Androl Dept Integrat Med, Nanjing 210009, Jiangsu, Peoples R China

[2] Qinghai Univ, Med Coll, Xining 810001, Qinghai, Peoples R China

[3] Hebei Prov Hosp Tradit Chinese Med, Shijiazhuang 050011, Hebei, Peoples R China

[4] Nanjing Med Univ, Affiliated Hosp 1, Clin Ctr Reprod Med, State Key Lab Reprod Med, Nanjing 210029, Jiangsu, Peoples R China

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2018年 / 41卷 / 09期

关键词：

Yangjing Capsule (YC); Leydig cell; apoptosis; testosterone; steroidogenic acute regulatory protein (StAR); TRANSCRIPTION;

D O I：

10.1248/bpb.b18-00205

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The present study aims to investigate the roles of steroidogenic acute regulatory protein (StAR) in Yangjing Capsule (YC) induced anti-apoptotic effects on Leydig cells and the related mechanism. Leydig tumor cells (MLTC-1) were cultured and treated with YC, and immunofluorescence assay was performed to examine the expression of StAR; furthermore, luciferase reporter assay was conducted to evaluate the impact of YC on StAR promoter; next, MLTC-1 cells were treated with StAR small interfering RNA (siRNA), and flow cytometry was carried out to examine the effect of StAR siRNA on the apoptosis of the cells; furthermore, quantitative (q)RT-PCR and Western blot methods was used to determine the expression of StAR and apoptosis related molecules Bcl-2, Bax and Caspase-3 on both mRNA and protein levels in different groups; finally the secretion of testosterone in different groups was examined by radioimmunoassay. We observed that the YC can increase the expression of StAR in a dose-dependent manner, and YC can activate the promoter of StAR; moreover, transfection of StAR siRNA can block YC induced anti-apoptotic effects and increased production of testosterone. In conclusion, our results suggested that YC might suppress the apoptosis of MLTC-1 cells and enhance the production of testosterone through regulating the expression of StAR.

引用

页码：1401 / 1405

页数：5

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