Selective DNA Delivery to Tumor Cells Using an Oligoarginine-LTVSPWY Peptide

被引：11

作者：

Gong, Cheng ^{[1
,2
]}

Pan, Deng ^{[1
,2
]}

Qiu, Fengwu ^{[1
,2
]}

Sun, Pei ^{[1
,2
]}

Zhang, Yu-Hui ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Dept Biomed Engn, Minist Educ, Key Lab Biomed Photon, Wuhan 430074, Hubei, Peoples R China

来源：

PLOS ONE | 2014年 / 9卷 / 10期

基金：

中国国家自然科学基金;

关键词：

GENE-DELIVERY; VIRAL VECTORS; IN-VIVO; MECHANISM; NANOPARTICLES; THERAPEUTICS; OCTAARGININE; EXPRESSION; THERAPY; DESIGN;

D O I：

10.1371/journal.pone.0110632

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA therapy for cancer requires efficient, selective and safe DNA delivery systems. Compared with other non-viral methods such as lipid or polymer-based DNA delivery vectors, peptide-based DNA delivery systems are biocompatible and biodegradable, which leads to lower immunogenicity and lower toxicity. Moreover, peptide vectors are easier to produce and their compositions easier to control because solid-phase peptide synthesis has been extensively developed. However, peptide-based systems for DNA delivery toward special tumor cells or tissues are still lacking. In this study, we constructed a non-viral 9rR-LTVSPWY peptide-based DNA delivery system and showed that it is able to efficiently and selectively transfect DNA into targeted tumor cells. This work presents a novel strategy for tumor cell-specific DNA delivery and a reference for designing more efficient DNA delivery systems targeted towards various types of cancer.

引用

页数：7

共 34 条

[1] Physical stability of proteins in aqueous solution: Mechanism and driving forces in nonnative protein aggregation [J].