Identification of microorganisms grown on chromogenic media by MALDI-TOF MS

被引：7

作者：

Luthje, Petra ^{[1
]}

Pranada, Arthur B. ^{[2
]}

Carruthers-Lay, Duncan ^{[3
]}

Desjardins, Marc ^{[3
]}

Gaillot, Olivier ^{[4
]}

Wareham, David ^{[5
,6
]}

Ciesielczuk, Holly ^{[6
]}

Ozenci, Volkan ^{[1
]}

机构：

[1] Karolinska Univ, Hosp Huddinge, Karolinska Inst, Div Clin Microbiol, Stockholm, Sweden

[2] MW Dr Eberhard Ei Partner Dortmund UBAG, Dortmund, Germany

[3] Ottawa Hosp, Ottawa, ON, Canada

[4] Ctr Hosp Reg Univ Lille, Lille, France

[5] Queen Mary Univ London, London, England

[6] Barts Hlth NHS Trust, Div Infect, London, England

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 136卷

关键词：

Bacterial identification; Chromogenic media; Culture conditions; MALDI-TOF MS; FLIGHT MASS-SPECTROMETRY; DESORPTION IONIZATION-TIME; ROUTINE IDENTIFICATION; BRUKER BIOTYPER; OF-FLIGHT; BACTERIA;

D O I：

10.1016/j.mimet.2017.03.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and chromogenic media are widely used in clinical microbiology laboratories to facilitate the rapid selection and identification of pathogens. The aim of this study was to evaluate whether usage of chromogenic media limits the diagnostic performance of MALDI-TOF MS for microbial identification. A total of 386 microorganisms collected and analyzed at five laboratories were included. Isolates were cultured on relevant chromogenic media and non-selective agar plates in parallel and identified using the Bruker MALDI-TOF MS. Among the tested isolates, no misidentification was recorded and there was no medium-related difference in the identification level. However, score values were overall slightly but significantly lower for isolates grown on chromogenic media. In conclusion, the use of chromogenic culture media tested here had no relevant impact on MALDI-TOF MS performance for diagnostic purposes. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：17 / 20

页数：4

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