Structure characterization and hypoglycemic activity of a polysaccharide isolated from the fruit of Lycium barbarum L.

被引：167

作者：

Zou, Shan ^{[1
]}

Zhang, Xian ^{[1
]}

Yao, Wenbing ^{[1
]}

Niu, Yuge ^{[1
]}

Gao, Xiangdong ^{[1
]}

机构：

[1] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 80卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Lycium barbarm L; Polysaccharide; Structure analysis; Hypoglycemic activity; PECTIC POLYSACCHARIDE; ANTIOXIDANT ACTIVITY; FT-IR; METHYLATION; EXTRACTS; SKIN;

D O I：

10.1016/j.carbpol.2010.01.038

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An acidic polysaccharide LBP-1 isolated from the fruit of Lycium barbarum L. was purified by ion-exchanged column. Structure characterization and in vitro hypoglycemic activity of LBP-1 were determined. Chemical analysis indicated LBP-1 was composed with rhamnose, arabinose, xylose, galactose, mannose, galacturonic acid = 1.00:7.85:0.37:0.65:3.01:8.16 with average molecular weight of 2.25 x 10(6) Da. The structure features of LBP-1 were investigated by FT-IR, GC-MS, H-1-NMR and C-13-NMR. The backbone were mainly composed of (1,5)-linkage arabinose, (1,4)-linkage galacturonic acid, -(1)-mannose-(3,6)-linkage and terminated with -(1)-mannose. LBP-1 protected the pancreatic islets cells from oxidative damage and enhanced cell survival ratio significantly. Moreover, its application inhibited the development of insulin resistance in HepG2 cells. This paper indicates the beneficial effect of LBP-1 and explores a potential hypoglycemic functional food and pharmaceuticals with definitely structure. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1161 / 1167

页数：7

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