A genetic linkage map of marine shrimp Penaeus (Fenneropenaeus) chinensis based on AFLP, SSR, and RAPD markers

被引:14
|
作者
Liu Bo [1 ,2 ]
Wang Qingyin [1 ]
Li Jian [1 ]
Liu Ping [1 ]
He Yuying [1 ]
机构
[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
来源
关键词
AFLP; linkage map; Penaeus (Fenneropenaeus) chinensis; RAPD; SSR; HALIOTIS-DISCUS-HANNAI; LITOPENAEUS-VANNAMEI; MICROSATELLITE; CONSTRUCTION; DISTORTION;
D O I
10.1007/s00343-010-9915-3
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Chinese shrimp Penaeus (Fenneropaeneus) chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp Penaeus (Fenneropaeneus) chinensis was captured from west coast of the Korean peninsula and mated with a "Yellow Sea No. 1" male to produce the first filial generation (F-1) 100 F-2 full-sib progeny from brother-sister crosses between F-1 families was used for the mapping study. A genetic linkage map of the Chinese shrimp was constructed, based on 354 markers, including 300 amplified fragment length polymorphism (AFLP) markers, 42 microsatellite (SSR) markers, and 12 randomly amplified polymorphism (RAPD) markers. Forty-seven linkage groups (LGs) were identified. The total map length was 4 580.5 cM, with an average spacing of 11.3 cM, covering 75.8% of the estimated genome size. The construction of this genetic linkage map was part of a genetic breeding program. This linkage map will contribute to the discovery of genes and quantitative trait loci (QTLs) in Chinese shrimp.
引用
收藏
页码:815 / 825
页数:11
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