PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER)

被引：44

作者：

Chen, Li-Zhen ^{[1
]}

Liang, Ge-Mei ^{[1
]}

Zhang, Jie ^{[1
]}

Wu, Kong-Ming ^{[1
]}

Guo, Yu-Yuan ^{[1
]}

Rector, Brian G. ^{[2
]}

机构：

[1] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

[2] USDA ARS, European Biol Control Lab, Montpellier, France

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 2010年 / 73卷 / 02期

关键词：

Bacillus thuringiensis; Helicoverpa armigera; brush border membrane vesicles; proteomics; peptide mass fingerprinting; HELIOTHIS-VIRESCENS LARVAE; BORDER MEMBRANE-VESICLES; AEDES-AEGYPTI LARVAE; BACILLUS-THURINGIENSIS; ALKALINE-PHOSPHATASE; INSECT RESISTANCE; GENE CLONING; TOXIN; RECEPTOR; MIDGUT;

D O I：

10.1002/arch.20340

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aminopeptidase N (APN) and cadherin- like proteins have been previously identified as Cry1Ac- binding proteins in Helicoverpa armigera (Hu " bner). In this study, a proteomic approach was used to identify novel Cry1Ac- binding proteins in H. armigera. Brush border membrane vesicles (BBMV) of H. armigera were extracted and separated by two- dimensional gel electrophoresis (2- DE). Cry1Ac- binding proteins were detected using antisera against Cry1Ac. Peptide mass fingerprinting (PMF) was used to identify Cry1Ac- binding proteins. In total, four proteins were identified as candidate Cry1Ac- binding proteins in H. armigera: vacuolar ATP synthase (V- ATPase) subunit B, actin, heat shock cognate protein (HSCP), and a novel protein. (C) 2009 Wiley Periodicals, Inc.

引用

页码：61 / 73

页数：13

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