Improved specificity of reagentless amperometric PQQ-sGDH glucose biosensors by using indirectly heated electrodes

被引:40
|
作者
Lau, Carolin
Borgmann, Sabine
Maciejewska, Monika
Ngounou, Bertrand
Gruendler, Peter
Schuhmann, Wolfgang
机构
[1] Univ Rostock, D-18051 Rostock, Germany
[2] Ruhr Univ Bochum, D-44780 Bochum, Germany
[3] Wroclaw Univ Technol, PL-50377 Wroclaw, Poland
来源
BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 12期
关键词
heated electrode; heated biosensor; PQQ-sGDH; interference elimination; reagentless biosensor; thermal discrimination;
D O I
10.1016/j.bios.2006.12.033
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Indirectly heated electrodes operating in a non-isothermal mode have been used as transducers for reagentless glucose biosensors. PyrToloquinoline quinone-dependent soluble glucose dehydrogenase (PQQ-sGDH) was entrapped on the electrode surface within a redox hydrogel layer. Localized polymer film precipitation was invoked by electrochemically modulating the pH-value in the diffusion zone in front of the electrode. The resulting decrease in solubility of an anodic electrodeposition paint (EDP) functionalized. with Osmium complexes leads to precipitation of the redox hydrogel concomitantly entrapping the enzyme. The resulting sensor architecture enables a fast electron transfer between enzyme and electrode surface. The glucose sensor was operated at pre-defined temperatures using a multiple current-pulse mode allowing reproducible indirect heating of the sensor. The sensor characteristics such as the apparent Michaelis constants K-M(app) and maximum currents I-max(app) were determined at different temperatures for the main substrate glucose as well as a potential interfering co-substrate maltose. The limit of detection increased with higher temperatures for both substrates (0.020 mM for glucose, and 0.023 mM for maltose at 48 degrees C). The substrate specificity of PQQ-sGDH is highly temperature dependent. Therefore, a mathematical model based on a multiple linear regression approach could be applied to discriminate between the current response for glucose and maltose. This allowed accurate determination of glucose in a concentration range of 0-0.1 mM in the presence of unknown maltose concentrations ranging from 0 to 0.04 MM. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:3014 / 3020
页数:7
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