Modified chitosan encapsulated core-shell Ag Nps for superior antimicrobial and anticancer activity

被引：40

作者：

Banerjee, Sovan Lal ^{[1
,2
]}

Khamrai, Moumita ^{[1
]}

Sarkar, Kishor ^{[1
]}

Singha, Nikhil K. ^{[2
]}

Kundu, P. P. ^{[1
]}

机构：

[1] Univ Calcutta, Univ Coll Sci & Technol, Dept Polymer Sci & Technol, 92 APC Rd, Kolkata 700009, W Bengal, India

[2] Indian Inst Technol, Ctr Rubber Technol, Kharagpur 721302, W Bengal, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 85卷

关键词：

Chitosan-g-poly(acrylamide); Polyethylene glycol; Silver nanoparticles; Antimicrobial activity; Cytotoxicity; SILVER NANOPARTICLES; MOLECULAR-WEIGHT; ALKYLATED POLYETHYLENIMINES; GREEN SYNTHESIS; DRUG-DELIVERY; IN-SITU; ANTIBACTERIAL; GOLD; NANOCOMPOSITE; COPOLYMER;

D O I：

10.1016/j.ijbiomac.2015.12.068

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This investigation reports a one pot synthesis of silver nanoparticles (Ag Nps) using aqueous solution of chitosan-graft-poly(acrylamide) (Cts-g-PAAm) as a reducing agent and polyethylene glycol (PEG) as a stabilizing agent. The as synthesized Ag Nps was characterized by ultra violet-visible (UV-vis), Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analysis. Field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS) and transmission electron microscopy (TEM) showed that Ag Nps, which were stable upto more than 60 days, were spherical in shape and the particle size was in the range of 5-50 nm. Atomic force microscopy (AFM) image also supported the above obtained result. The prepared Ag Nps exhibited strong antimicrobial activity against different gram positive bacteria (Alkaliphilus, Bascillus substillis, Lysinibascillus) and gram negative bacteria (Enterobacter aerogenus, Vivbrio vulnificus and Escherichia coli) and haemolytic assay revealed its blood compatible nature. The synthesized Ag Nps showed significant cytotoxicity over human cervical HeLa cancer cells and it was found that the inhibitory concentration for 50% cell death (IC50) was 8 mu g/ml. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：157 / 167

页数：11

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