Cytotoxic Induction and Photoacoustic Imaging of Breast Cancer Cells Using Astaxanthin-Reduced Gold Nanoparticles

被引：26

作者：

Bharathiraja, Subramaniyan ^{[1
,2
]}

Manivasagan, Panchanathan ^{[1
,2
]}

Bui, Nhat Quang ^{[1
,3
]}

Oh, Yun-Ok ^{[1
,2
]}

Lim, In Gweon ^{[4
]}

Park, Suhyun ^{[5
]}

Oh, Junghwan ^{[1
,2
,3
]}

机构：

[1] Pukyong Natl Univ, Dept Biomed Engn, Busan 608737, South Korea

[2] Pukyong Natl Univ, Marine Integrated Bion Res Ctr, Busan 608737, South Korea

[3] Pukyong Natl Univ, Ctr Marine Integrated Biotechnol Plus BK21, Busan 608737, South Korea

[4] Myong Ji Univ, Dept Mech Engn, San 38-2, Yongin 449728, Gyeonggi Do, South Korea

[5] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

来源：

NANOMATERIALS | 2016年 / 6卷 / 04期

关键词：

astaxanthin; breast cancer; gold nanoparticle; cytotoxicity; photoacoustic imaging; BIOMEDICAL APPLICATIONS; SILVER NANOPARTICLES; APOPTOSIS; ANTIOXIDANT;

D O I：

10.3390/nano6040078

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Astaxanthin, a kind of photosynthetic pigment, was employed for gold nanoparticle formation. Nanoparticles were characterized using Ulteraviolet-Visible (UV-Vis) spectroscopy, transmission electron microscopy, and X-ray diffraction, and the possible presence of astaxanthin functional groups were analyzed by Fourier transform infrared spectroscopy (FTIR). The cytotoxic effect of synthesized nanoparticles was evaluated against MDA-MB-231 (human breast cancer cells) using a tetrazolium-based assay, and synthesized nanoparticles exhibited dose-dependent toxicity. The morphology upon cell death was differentiated through fluorescent microscopy using different stains that predicted apoptosis. The synthesized nanoparticles were applied in ultrasound-coupled photoacoustic imaging to obtain good images of treated cells. Astaxanthin-reduced gold nanoparticle has the potential to act as a promising agent in the field of photo-based diagnosis and therapy.

引用

页数：11

共 31 条

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