A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research

被引：44
|
作者：
Ohashi, Hiroyuki [1 ,2 ]
Kanamori, Takashi [1 ]
Shimizu, Yoshihiro [1 ]
Ueda, Takuya [1 ]
机构：
[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Chiba 2778562, Japan
[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England
来源：
CURRENT PHARMACEUTICAL BIOTECHNOLOGY | 2010年 / 11卷 / 03期
关键词：
Cell-free protein synthesis; in vitro translation; chaperone; disulfide bond formation; in vitro selection; unnatural amino acid; FREE TRANSLATION SYSTEM; PURIFIED COMPONENTS; NASCENT CHAIN;
D O I：
10.2174/138920110791111889
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
引用
收藏
页码：267 / 271
页数：5
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