A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research

被引：44
|
作者：
Ohashi, Hiroyuki [1 ,2 ]
Kanamori, Takashi [1 ]
Shimizu, Yoshihiro [1 ]
Ueda, Takuya [1 ]
机构：
[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Chiba 2778562, Japan
[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England
来源：
CURRENT PHARMACEUTICAL BIOTECHNOLOGY | 2010年 / 11卷 / 03期
关键词：
Cell-free protein synthesis; in vitro translation; chaperone; disulfide bond formation; in vitro selection; unnatural amino acid; FREE TRANSLATION SYSTEM; PURIFIED COMPONENTS; NASCENT CHAIN;
D O I：
10.2174/138920110791111889
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
引用
收藏
页码：267 / 271
页数：5
相关论文
共 50 条
  • [1] Integration of a Reconstituted Cell-free Protein-synthesis System on a Glass Microchip
    Tanaka, Yo
    Shimizu, Yoshihiro
    ANALYTICAL SCIENCES, 2015, 31 (02) : 67 - 71
  • [2] Integration of a Reconstituted Cell-free Protein-synthesis System on a Glass Microchip
    Yo Tanaka
    Yoshihiro Shimizu
    Analytical Sciences, 2015, 31 : 67 - 71
  • [3] Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system
    Satoshi Murakami
    Rena Matsumoto
    Takashi Kanamori
    Scientific Reports, 9
  • [4] Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system
    Murakami, Satoshi
    Matsumoto, Rena
    Kanamori, Takashi
    SCIENTIFIC REPORTS, 2019, 9 (1)
  • [5] Epitope Mapping Using Ribosome Display in a Reconstituted Cell-Free Protein Synthesis System
    Osada, Eriko
    Shimizu, Yoshihiro
    Akbar, Bintang K.
    Kanamori, Takashi
    Ueda, Takuya
    JOURNAL OF BIOCHEMISTRY, 2009, 145 (05): : 693 - 700
  • [6] Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs
    Hibi, Keita
    Amikura, Kazuaki
    Sugiura, Naoki
    Masuda, Keiko
    Ohno, Satoshi
    Yokogawa, Takashi
    Ueda, Takuya
    Shimizu, Yoshihiro
    COMMUNICATIONS BIOLOGY, 2020, 3 (01)
  • [7] Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs
    Keita Hibi
    Kazuaki Amikura
    Naoki Sugiura
    Keiko Masuda
    Satoshi Ohno
    Takashi Yokogawa
    Takuya Ueda
    Yoshihiro Shimizu
    Communications Biology, 3
  • [8] G-Protein Coupled Receptor Protein Synthesis on a Lipid Bilayer Using a Reconstituted Cell-Free Protein Synthesis System
    Gessesse, Belay
    Nagaike, Takashi
    Nagata, Koji
    Shimizu, Yoshihiro
    Ueda, Takuya
    LIFE-BASEL, 2018, 8 (04):
  • [9] A highly efficient cell-free protein synthesis system from Escherichia coli
    Kim, DM
    Kigawa, T
    Choi, CY
    Yokoyama, S
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 239 (03): : 881 - 886
  • [10] NITROGEN ASSIMILATION BY A CELL-FREE RECONSTITUTED SYSTEM
    GRAY, VM
    CRESSWELL, CF
    SOUTH AFRICAN JOURNAL OF BOTANY, 1982, 1 (03) : 80 - 81
12345