Ligninolytic enzyme production and dye decolorization by Trametes trogii:: application of the Plackett-Burman experimental design to evaluate nutritional requirements

被引：102

作者：

Levin, L ^{[1
]}

Forchiassin, F

Viale, A

机构：

[1] Univ Buenos Aires, Dpto Biodivers & Biol Expt, RA-1428 Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Biol, RA-1428 Buenos Aires, DF, Argentina

来源：

PROCESS BIOCHEMISTRY | 2005年 / 40卷 / 3-4期

关键词：

Plackett-Burman design; ligninolytic enzymes; dye decolorization; nutritional requirements; white rot fungi; Trametes trogii;

D O I：

10.1016/j.procbio.2004.06.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of medium components on laccase, manganese peroxidase and glyoxal oxidase production by Trametes trogii (MYA 28-11) have been studied using Plackett-Burman (PB) experimental design. Copper had the highest positive influence on ligninolytic enzyme production. T trogii extracellular fluids obtained in the medium which rendered the highest ligninolytic production (45.32 U/ml laccase, 214.5 mU/ml manganese peroxidase and 116 mU/ml glyoxal oxidase) also showed the greatest ability to decolorize the dyes Ponceau 2R (a xylidine derivative), malachite green and anthraquinone blue (at rates of 2.14, 1.35 mug and 3 mg dye/(ml h), respectively). The relationship among decolorization rates and ligninolytic enzyme activities was analyzed by multiple regression. The results fit a linear plus interactions model. The comparison of the response surfaces obtained, suggests that while laccase activity has a greater importance in xylidine degradation, manganese peroxidase activity plays the major role in malachite green decolorization. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1381 / 1387

页数：7

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