Adenovirus vector-mediated gene transfer into rat retinal neurons and Muller cells in vitro and in vivo

被引：10
作者：
Fukuhara, M
Suzuki, A
Fukuda, Y
Kosaka, J
机构：
[1] Osaka Univ, Sch Med, Dept Physiol, Osaka 565, Japan
[2] Osaka Med Coll, Dept Ophthalmol, Osaka 56911, Japan
来源：
NEUROSCIENCE LETTERS | 1998年 / 242卷 / 02期
关键词：
adenovirus vector; CAG promoter; gene transfer; Muller cells; retina; rat;
D O I：
10.1016/S0304-3940(98)00055-X
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The ability and efficiency of human type V adenovirus-originated vector with CAG promoter to transfer foreign genes was examined in the rat retina. We introduced the adenovirus vector, AxCALacZ with the reporter gene LacZ (beta-galactosidase gene) into cultured retinal cells, and then injected the vector suspension into the vitreous cavity of the eye. Beta-galactosidase staining was observed in both glial and neuronal cells in vitro and in Muller cells near the injection site in vivo. Adenovirus vectors with CAG promoter are useful and efficient for the expression of foreign genes in retinal cells. (C) 1998 Elsevier Science Ireland Ltd.
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收藏
页码：93 / 96
页数：4
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