An optimized protocol for analysis of EST sequences

被引：99

作者：

Liang, F ^{[1
]}

Holt, I ^{[1
]}

Pertea, G ^{[1
]}

Karamycheva, S ^{[1
]}

Salzberg, SL ^{[1
]}

Quackenbush, J ^{[1
]}

机构：

[1] Inst Genome Res, Rockville, MD 20850 USA

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 18期

关键词：

D O I：

10.1093/nar/28.18.3657

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The vast body of Expressed Sequence Tag (EST) data in the public databases provide an important resource for comparative and functional genomics studies and an invaluable tool for the annotation of genomic sequences. We have developed a rigorous protocol for reconstructing the sequences of transcribed genes from EST and gene sequence fragments. A key element in developing this protocol has been the evaluation of a number of sequence assembly programs to determine which most faithfully reproduce transcript sequences from EST data. The TIGR Gene Indices constructed using this protocol for human, mouse, rat and a variety of other plant and animal models have demonstrated their utility in a variety of applications and are freely available to the scientific research community.

引用

页码：3657 / 3665

页数：9

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