Third harmonic generation imaging for fast, label-free pathology of human brain tumors

被引：70

作者：

Kuzmin, N. V. ^{[1
,2
]}

Wesseling, P. ^{[3
,4
,6
]}

Hamer, P. C. de Witt ^{[5
,6
]}

Noske, D. P. ^{[5
,6
]}

Galgano, G. D. ^{[1
]}

Mansvelder, H. D. ^{[2
]}

Baayen, J. C. ^{[5
]}

Groot, M. L. ^{[1
,2
]}

机构：

[1] Vrije Univ Amsterdam, LaserLab Amsterdam, De Boelelaan 1081, NL-1081 HV Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, Neurosci Campus Amsterdam, De Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands

[3] Vrije Univ Amsterdam Med Ctr, Dept Pathol, De Boelelaan 1117, NL-1081 HV Amsterdam, Netherlands

[4] Radboud Univ Nijmegen Med Ctr, Dept Pathol, Geert Grootepl Zuid, NL-6525 GA Nijmegen, Netherlands

[5] Vrije Univ Amsterdam Med Ctr, Dept Neurosurg, De Boelelaan 1117, NL-1081 HV Amsterdam, Netherlands

[6] Vrije Univ Amsterdam Med Ctr, Amsterdam Brain Tumor Ctr, De Boelelaan 1117, NL-1081 HV Amsterdam, Netherlands

来源：

BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 05期

关键词：

3RD-HARMONIC GENERATION; MULTIPHOTON MICROSCOPY; VIVO; FLUORESCENCE; RESECTION; GLIOMA; TISSUES; CANCER; MECHANISMS; EMBRYOS;

D O I：

10.1364/BOE.7.001889

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative assessment of tumor boundaries by the neurosurgeon is difficult. Therefore, there is an urgent need for tools that provide the neurosurgeon with pathological information during the operation. We show that third harmonic generation (THG) microscopy provides label-free, real-time images of histopathological quality; increased cellularity, nuclear pleomorphism, and rarefaction of neuropil in fresh, unstained human brain tissue could be clearly recognized. We further demonstrate THG images taken with a GRIN objective, as a step toward in situ THG microendoscopy of tumor boundaries. THG imaging is thus a promising tool for optical biopsies. (C) 2016 Optical Society of America

引用

页码：1889 / 1904

页数：16

共 56 条

[1] Optical Biomarkers of Serous and Mucinous Human Ovarian Tumor Assessed with Nonlinear Optics Microscopies [J].