Cellular Engineering with Membrane Fusogenic Liposomes to Produce Functionalized Extracellular Vesicles

被引：118

作者：

Lee, Junsung ^{[1
,2
,3
]}

Lee, Hyoungjin ^{[1
,3
]}

Goh, Unbyeol ^{[1
,3
]}

Kim, Jiyoung ^{[1
,3
]}

Jeong, Moonkyoung ^{[1
,3
]}

Lee, Jean ^{[1
,3
]}

Park, Ji-Ho ^{[1
,2
,3
,4
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Bio & Brain Engn, Daejeon 34141, South Korea

[2] Korea Adv Inst Sci & Technol, Grad Sch Med Sci & Engn, Daejeon 34141, South Korea

[3] Korea Adv Inst Sci & Technol, Inst Hlth Sci & Technol, Daejeon 34141, South Korea

[4] Korea Adv Inst Sci & Technol, Inst Nanocentury, Daejeon 34141, South Korea

来源：

ACS APPLIED MATERIALS & INTERFACES | 2016年 / 8卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

cellular engineering click chemistry; drug delivery; extracellular vesicle; liposome; BREAST-CANCER CELLS; EXOSOMES; DELIVERY; ELECTROPORATION; STABILITY; MECHANISM; THERAPY; BRAIN; SIRNA;

D O I：

10.1021/acsami.6b01315

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Engineering of extracellular vesicles (EVs) without affecting biological functions remains a challenge, limiting the broad applications of EVs in biomedicine. Here, we report a method to equip EVs with various functional agents, including fluorophores, drugs, lipids, and bio-orthogonal chemicals, in an efficient and controlled manner by engineering parental cells with membrane fusogenic liposomes, while keeping the EVs intact. As a demonstration of how this method can be applied, we prepared EVs containing azide-lipids, and conjugated them with targeting peptides using copper-free click chemistry to enhance targeting efficacy to cancer cells. We believe that this liposome-based cellular engineering method will find utility in studying the biological roles of EVs and delivering therapeutic agents through their innate pathway.

引用

页码：6790 / 6795

页数：6

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