Toward investigating changes in cell mechanoelastic properties in response to nanosecond pulsed electric fields

被引：0

作者：

Coker, Zachary ^{[1
,2
]}

Troyanova-Wood, Maria ^{[1
]}

Traverso, Andrew ^{[1
]}

Meng, Zhaokai ^{[1
]}

Ballmann, Charles ^{[1
]}

Petrov, Georgi ^{[1
]}

Ibey, Bennett L. ^{[2
]}

Yakovlev, Vladislav ^{[1
]}

机构：

[1] Texas A&M Univ, 3120 TAMU, College Stn, TX 77843 USA

[2] Air Force Res Lab, Human Performance Wing 711, Human Effectiveness Directorate, Radio Frequency Bioeffects Branch,Bioeffects Div, Jbsa Ft Sam Houston, TX 78234 USA

来源：

ENERGY-BASED TREATMENT OF TISSUE AND ASSESSMENT IX | 2017年 / 10066卷

基金：

美国国家科学基金会;

关键词：

Brillouin Spectroscopy; mechanoelasticity; nanosecond electric pulse (nsEP); nanoporation; CHO-K1; cells; electroporation; MEMBRANE PERMEABILIZATION; BRILLOUIN;

D O I：

10.1117/12.2253451

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Nanosecond electric pulses (nsEPs) are known to cause a variety of effects on mammalian cells, ranging from destabilization of cell membranes to changes in cytoskeleton and elastic moduli. Measurement of a cells mechanoelastic properties have previously been limited to only invasive and destructive techniques such as atomic force microscopy or application of optical tweezers. However, due to recent advances, Brillouin spectroscopy has now become viable as a non-contact, non-invasive method for measuring these properties in cells and other materials. Here, we present progress toward applying Brillouin spectroscopy using a unique microscopy system for measuring changes in CHO-K1 cells when exposed to nsEPs of 600ns pulse duration with intensity of 50kV/cm. Successful measurement of mechanoelastic changes in these cells will demonstrate Brillouin spectroscopy as a viable method for measuring changes in elastic properties of other cells and living organisms.

引用

页数：6

共 27 条

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