Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp. BH72, a grass-associated diazotroph, on rice roots

被引：60

作者：

Egener, T ^{[1
]}

Hurek, T ^{[1
]}

Reinhold-Hurek, B ^{[1
]}

机构：

[1] Max Planck Inst Terr Mikrobiol, Arbeitsgrp Symbioseforsch, D-35043 Marburg, Germany

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1998年 / 11卷 / 01期

关键词：

nitrogen fixation;

D O I：

10.1094/MPMI.1998.11.1.71

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gfp (green fluorescent protein) cassette for transcriptional fusions has been developed to study gene expression in Azoarcus sp. BH72 in association with plant roots. The bacteria expressed nitrogenase genes (nifHDK) in the rhizosphere, on root tips, and in epidermal cells of rice seedlings. Green fluorescent protein fusions also visualized promoter activity of single cells in soil.

引用

页码：71 / 75

页数：5

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