Antigenic and Pathogenic Characteristics of QX-Type Avian Infectious Bronchitis Virus Strains Isolated in Southwestern China

被引：23

作者：

Li, Shuyun ^{[1
]}

Du, Lijing ^{[1
]}

Xia, Jing ^{[1
]}

Du, Jiteng ^{[1
]}

You, Guojin ^{[1
]}

Wen, Yiping ^{[1
]}

Huang, Xiaobo ^{[1
]}

Zhao, Qing ^{[1
]}

Han, Xinfeng ^{[1
]}

Yan, Qigui ^{[1
]}

Wu, Rui ^{[1
]}

Cui, Min ^{[1
]}

Cao, Sanjie ^{[1
]}

Huang, Yong ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Key Lab Anim Dis & Human Hlth Sichuan Prov, Huimin Rd 211, Chengdu 611130, Sichuan, Peoples R China

来源：

VIRUSES-BASEL | 2019年 / 11卷 / 12期

关键词：

infectious bronchitis virus; IBV; QX; antigenic; pathogenic; S1; GENOTYPE; EFFICACY; BINDING; GLYCOPROTEIN; DIVERSITY; EVOLUTION; TROPISM; PROTEIN; SCZY3;

D O I：

10.3390/v11121154

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The QX-type avian infectious bronchitis virus (IBV) is still a prevalent genotype in Southwestern China. To analyze the antigenicity and pathogenicity characteristics of the dominant genotype strains (QX-type), S1 gene sequence analysis, virus cross-neutralization tests, and pathogenicity test of eight QX-type IBV isolates were conducted. Sequence analysis showed that the nucleotide homology between the eight strains was high, but distantly related to H120 and 4/91 vaccine strains. Cross-neutralization tests showed that all eight strains isolated from 2015 and 2017 belonged to the same serotype, but exhibited antigenic variations over time. The pathogenicity test of the five QX-type IBV isolates showed that only three strains, CK/CH/SC/DYW/16, CK/CH/SC/MS/17, and CK/CH/SC/GH/15, had a high mortality rate with strong respiratory and renal pathogenicity, whereas CK/CH/SC/PZ/17 and CK/CH/SC/DYYJ/17 caused only mild clinical symptoms and tissue lesions. Our results indicate that the prevalent QX-type IBVs displayed antigenic variations and pathogenicity difference. These findings may provide reference for research on the evolution of IBV and vaccine preparation of infectious bronchitis (IB).

引用

页数：14

共 39 条

[1] Characterization and analysis of the full-length genome of a strain of the European QX-like genotype of infectious bronchitis virus [J].

Abro, Shahid Hussain ;

Renstrom, Lena H. M. ;

Ullman, Karin ;

Belak, Sandor ;

Baule, Claudia .

ARCHIVES OF VIROLOGY, 2012, 157 (06) :1211-1215

[2] Isolation, propagation, identification and comparative pathogenicity of five Egyptian field strains of Eimeria tenella from broiler chickens in five different provinces in Egypt [J].

Abu-Akkada, Somaia Saif ;

Awad, Ashraf Mahmoud .

RESEARCH IN VETERINARY SCIENCE, 2012, 92 (01) :92-95

[3] EFFECT OF PH ON GROWTH AND CYTOPATHOGENICITY OF AVIAN INFECTIOUS BRONCHITIS VIRUS IN CHICK KIDNEY-CELLS [J].

ALEXANDER, DJ ;

COLLINS, MS .

ARCHIVES OF VIROLOGY, 1975, 49 (04) :339-348

[4] Comparative features of infections of two Massachusetts (Mass) infectious bronchitis virus (IBV) variants isolated from Western Canadian layer flocks [J].

Amarasinghe, Aruna ;

Senapathi, Upasama De Silva ;

Abdul-Cader, Mohamed Sarjoon ;

Popowich, Shelly ;

Marshall, Frank ;

Cork, Susan C. ;

van der Meer, Frank ;

Gomis, Susantha ;

Abdul-Careem, Mohamed Faizal .

BMC VETERINARY RESEARCH, 2018, 14

[5] Progress and Challenges toward the Development of Vaccines against Avian Infectious Bronchitis [J].

Bande, Faruku ;

Arshad, Siti Suri ;

Bejo, Mohd Hair ;

Moeini, Hassan ;

Omar, Abdul Rahman .

JOURNAL OF IMMUNOLOGY RESEARCH, 2015, 2015

[6] AntigenMap 3D: an online antigenic cartography resource [J].

Barnett, J. Lamar ;

Yang, Jialiang ;

Cai, Zhipeng ;

Zhang, Tong ;

Wan, Xiu-Feng .

BIOINFORMATICS, 2012, 28 (09) :1292-1293

[7] CORONAVIRUS IBV - VIRUS RETAINING SPIKE GLYCOPOLYPEPTIDE-S2 BUT NOT S1 IS UNABLE TO INDUCE VIRUS-NEUTRALIZING OR HEMAGGLUTINATION-INHIBITING ANTIBODY, OR INDUCE CHICKEN TRACHEAL PROTECTION [J].

CAVANAGH, D ;

DAVIS, PJ ;

DARBYSHIRE, JH ;

PETERS, RW .

JOURNAL OF GENERAL VIROLOGY, 1986, 67 :1435-1442

[8]

Cavanagh D, 1997, ARCH VIROL, V142, P629

[9] Pathogenicity and tissue tropism of infectious bronchitis virus is associated with elevated apoptosis and innate immune responses [J].

Chhabra, Rajesh ;

Kuchipudi, Suresh V. ;

Chantrey, Julian ;

Ganapathy, Kannan .

VIROLOGY, 2016, 488 :232-241

[10] Assessing the economic burden of avian infectious bronchitis on poultry farms in Brazil [J].

Colvero, L. P. ;

Villarreal, L. Y. B. ;

Torres, C. A. ;

Brendano, P. E. .

REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES, 2015, 34 (03) :993-999

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