Tissue-specific gene silencing by RNA interference in the whitefly Bemisia tabaci (Gennadius)

被引:115
作者
Ghanim, Murad
Kontsedalov, Svetlana
Czosnek, Henryk
机构
[1] Agr Res Org, Volcani Ctr, Inst Plant Protect, Dept Entomol, IL-50250 Bet Dagan, Israel
[2] Hebrew Univ Jerusalem, Fac Agr, Robert H Smith Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel
关键词
whitefly; Bemisia tabaci; RNA interference; midgut; salivary gland; genomics;
D O I
10.1016/j.ibmb.2007.04.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The hemipteran whitefly Bemisia tabaci (Gennadius) species complex and the plant viruses they transmit pose major constraints to vegetable and fiber production, worldwide. The whitefly tissue- and developmental-specific gene expression has not been exhaustively studied despite its economic importance. In 2002, a functional genomic project was initiated, which generated several thousands expressed sequence tags (ESTs) and their sequence. This project provides the basic information to design experiments aimed at understanding and manipulating whitefly gene expression. In this communication, for the first time we provide evidence that the RNA interference mechanism discovered in many organisms, including in Hemiptera, is active in B. tabaci. By injecting into the body cavity long double-stranded RNA (dsRNA) molecules, specifically directed against genes uniquely expressed in the midgut and salivary glands, we were able to significantly inhibit the expression of the targeted mRNA in the different organs. Gene expression levels in RNAi-silenced whiteflies were reduced up to 70% compared to whiteflies injected with buffer or with a green fluorescent protein (GFP)-specific dsRNA. Phenotypic effects were observed in B. tabaci ovaries following dsRNA targeting the whitefly Drosophila chickadee homologue. Disruption of whitefly gene expression opens the door to new strategies aimed at curbing down the deleterious effects of this insect pest to agriculture. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:732 / 738
页数:7
相关论文
共 26 条
[1]   RNA interference of the salivary gland nitrophorin 2 in the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae) by dsRNA ingestion or injection [J].
Araujo, R. N. ;
Santos, A. ;
Pinto, F. S. ;
Gontijo, N. F. ;
Lehane, M. J. ;
Pereira, M. H. .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2006, 36 (09) :683-693
[2]   Whitefly transmission of plant viruses [J].
Brown, JK ;
Czosnek, H .
ADVANCES IN BOTANICAL RESEARCH, VOL 36: PLANT VIRUS VECTOR INTERACTIONS, 2002, 36 :65-100
[3]   WHITEFLY BIOLOGY [J].
BYRNE, DN ;
BELLOWS, TS .
ANNUAL REVIEW OF ENTOMOLOGY, 1991, 36 :431-457
[4]   CHICKADEE ENCODES A PROFILIN REQUIRED FOR INTERCELLULAR CYTOPLASM TRANSPORT DURING DROSOPHILA OOGENESIS [J].
COOLEY, L ;
VERHEYEN, E ;
AYERS, K .
CELL, 1992, 69 (01) :173-184
[5]  
COSTA HS, 1991, ENTOMOL EXP APPL, V61, P211, DOI 10.1007/BF00187724
[6]   Functions of the ecdysone receptor isoform-A in the hemimetabolous insect Blattella germanica revealed by systemic RNAi in vivo [J].
Cruz, Josefa ;
Mane-Padros, Daniel ;
Belles, Xavier ;
Martin, David .
DEVELOPMENTAL BIOLOGY, 2006, 297 (01) :158-171
[7]   Terminal cytokinesis events uncovered after an RNAi screen [J].
Echard, A ;
Hickson, GRX ;
Foley, E ;
O'Farrell, PH .
CURRENT BIOLOGY, 2004, 14 (18) :1685-1693
[8]   Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].
Fire, A ;
Xu, SQ ;
Montgomery, MK ;
Kostas, SA ;
Driver, SE ;
Mello, CC .
NATURE, 1998, 391 (6669) :806-811
[9]   Evidence for transovarial transmission of tomato yellow leaf curl virus by its vector, the whitefly Bemisia tabaci [J].
Ghanim, M ;
Morin, S ;
Zeidan, M ;
Czosnek, H .
VIROLOGY, 1998, 240 (02) :295-303
[10]   Digestive, salivary, and reproductive organs of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)B type [J].
Ghanim, M ;
Rosell, RC ;
Campbell, LR ;
Czosnek, H ;
Brown, JK ;
Ullman, DE .
JOURNAL OF MORPHOLOGY, 2001, 248 (01) :22-40