Biological Applications at the Cutting Edge of Cryo-Electron Microscopy

被引：14

作者：

Dillard, Rebecca S. ^{[1
]}

Hampton, Cheri M. ^{[1
,4
]}

Strauss, Joshua D. ^{[1
]}

Ke, Zunlong ^{[1
,2
]}

Altomara, Deanna ^{[1
]}

Guerrero-Ferreira, Ricardo C. ^{[1
,5
]}

Kiss, Gabriella ^{[1
,6
]}

Wright, Elizabeth R. ^{[1
,3
,7
]}

机构：

[1] Emory Univ, Childrens Healthcare Atlanta, Div Pediat Infect Dis, Sch Med, Atlanta, GA 30322 USA

[2] Georgia Inst Technol, Sch Biol Sci, Atlanta, GA 30332 USA

[3] Emory Univ, Robert P Apkarian Integrated Electron Microscopy, Atlanta, GA 30322 USA

[4] Air Force Res Lab, Mat & Mfg Directorate, Wright Patterson AFB, OH 45433 USA

[5] Univ Basel, Ctr Cellular Imaging & NanoAnalyt C CINA, Biozentrum, Mattenstr 26, CH-4058 Basel, Switzerland

[6] Thermo Fisher Sci, 5350 NE Dawson Creek Dr, Hillsboro, OR 97124 USA

[7] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

来源：

MICROSCOPY AND MICROANALYSIS | 2018年 / 24卷 / 04期

关键词：

cryo-electron microscopy (cryo-EM); cryo-electron tomography (cryo-ET); cryo-correlative light and electron microscopy (cryo-CLEM); transmission electron microscopy (TEM); vitrification; phase plates; direct electron detectors; CRYO-EM STRUCTURE; RESOLUTION ELECTRON CRYOMICROSCOPY; FLUORESCENCE LIGHT-MICROSCOPY; FOCUS PHASE-CONTRAST; RAPID FREEZING SPRF; BEAM-INDUCED MOTION; MOLECULAR ARCHITECTURE; SAMPLE PREPARATION; ANGSTROM STRUCTURE; CORRELATIVE LIGHT;

D O I：

10.1017/S1431927618012382

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Cryo-electron microscopy (cryo-EM) is a powerful tool for macromolecular to near-atomic resolution structure determination in the biological sciences. The specimen is maintained in a near-native environment within a thin film of vitreous ice and imaged in a transmission electron microscope. The images can then be processed by a number of computational methods to produce three-dimensional information. Recent advances in sample preparation, imaging, and data processing have led to tremendous growth in the field of cryo-EM by providing higher resolution structures and the ability to investigate macromolecules within the context of the cell. Here, we review developments in sample preparation methods and substrates, detectors, phase plates, and cryo-correlative light and electron microscopy that have contributed to this expansion. We also have included specific biological applications.

引用

页码：406 / 419

页数：14

共 204 条

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