Coupling termination of transcription to messenger RNA maturation in yeast

被引：213

作者：

Birse, CE

Minvielle-Sebastia, L

Lee, BA

Keller, W

Proudfoot, NJ

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

[2] Univ Basel, Biozentrum, Dept Cell Biol, CH-4056 Basel, Switzerland

来源：

SCIENCE | 1998年 / 280卷 / 5361期

基金：

英国惠康基金;

关键词：

D O I：

10.1126/science.280.5361.298

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The direct association between messenger RNA (mRNA) 3'-end processing and the termination of transcription was established for the CYC1 gene of Saccharomyces cerevisiae. The mutation of factors involved in the initial cleavage of the primary transcript at the poly(A) site (RNA 14, RNA 15, and PCF11) disrupted transcription termination at the 3' end of the CYC1 gene. In contrast, the mutation of factors involved in the subsequent polyadenylation step (PAP1, FIP1, and YTH1) had little effect. Thus, cleavage factors link transcription termination of RNA polymerase II with pre-mRNA 3'-end processing.

引用

页码：298 / 301

页数：4

共 36 条

[1] PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I [J].