Ooplasmic injection of elongating spermatids for the treatment of non-obstructive azoospermia

We applied the technique of ooplasmic elongating spermatid injection to the treatment of non-obstructive azoospermia, Mature oocytes were injected,vith elongating spermatids isolated from testicular biopsy material obtained from 13 non-obstructed azoospermic men, Seventy-three oocytes were successfully injected with elongating spermatids and were then cultured for 36 h, At 13 h post-injection 68 oocytes were found to be activated and 52 of them were fertilized, Forty-one 2- to 4-cell stage embryos developed from normally fertilized oocytes were transferred. At least two embryos were transferred to each female partner, Two pregnancies were achieved. Elongating spermatid injection may have a role in the treatment of non-obstructive azoospermia.