Modulation of UVA light-induced oxidative stress by β-carotene, lutein and astaxanthin in cultured fibroblasts

The ability of beta-carotene, lutein or astaxanthin to protect against Uh-induced oxidative stress in rat kidney fibroblasts (NRK) was assessed. Activities of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), and changes in thiobarbituric acid reactive substances (TBARS) were measured as indices of oxidative stress. Exposure to UVA light at a dose intensity of 5.6 mW/cm(2) for 4 h resulted in a significant decrease in CAT and SOD activities and a significant increase in TEARS. No cytotoxicity, as indicated by lactate dehydrogenase (LDH) release, was observed, beta-Carotene (1 mu M), lutein (1 mu M) and astaxanthin (10 nM) protect against UVA light-induced oxidative stress in vitro with astaxanthin exhibiting superior protective properties. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.