The Polycyclic Aromatic Hydrocarbon (PAH) degradation activities and genome analysis of a novel strain Stenotrophomonas sp. Pemsol isolated from Mexico

被引：29

作者：

Elufisan, Temidayo O. ^{[1
,2
]}

Rodriguez-Luna, Isabel C. ^{[1
]}

Oyedara, Omotayo Opemipo ^{[3
]}

Sanchez-Varela, Alejandro ^{[1
]}

Hernandez-Mendoza, Armando ^{[4
]}

Dantan Gonzalez, Edgar ^{[5
]}

Paz-Gonzalez, Alma D. ^{[6
]}

Muhammad, Kashif ^{[6
]}

Rivera, Gildardo ^{[6
]}

Angel Villalobos-Lopez, Miguel ^{[7
]}

Guo, Xianwu ^{[1
]}

机构：

[1] Inst Politecn Nacl, Ctr Biotecnol Genom, Lab Biotecnol Genom, Reynosa, Tamaulipas, Mexico

[2] Obafemi Awolowo Univ, Natl Ctr Technol Management, SPIS, Campus Ile Ife, Ife, Osun, Nigeria

[3] Osun State Univ, Microbiol Dept, Osogbo, Osun, Nigeria

[4] UAEM, Inst Invest Ciencias Basicas & Aplicadas, Ctr Invest Dinam Celular, Cuernavaca, Morelos, Mexico

[5] Univ Autonoma Estado Morelos, Ctr Invest Biotecnol, Lab Estudios Ecogenom, Cuernavaca, Morelos, Mexico

[6] Inst Politecn Nacl, Ctr Biotecnol Genom, Lab Biotecnol Famaceut, Reynosa, Tamaulipas, Mexico

[7] Inst Politecn Nacl, Ctr Invest Biotecnol Aplicada, Tepetitla, Tlaxcala, Mexico

来源：

PEERJ | 2020年 / 8卷

关键词：

Stenotrophomonas; Sequencing; Biphenyl; Polycyclic Aromatic Hydrocarbon (PAH); Naphthalene; Degradation; MALTOPHILIA; BIODEGRADATION; NAPHTHALENE; SEQUENCE; ANNOTATION; BIOREMEDIATION; PHENANTHRENE; DIOXYGENASE; ADAPTATION; SALICYLATE;

D O I：

10.7717/peerj.8102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background. Stenotrophomonas are ubiquitous gram-negative bacteria, which can survive in a wide range of environments. They can use many substances for their growth and are known to be intrinsically resistant to many antimicrobial agents. They have been tested for biotechnological applications, bioremediation, and production of antimicrobial agents. Method. Stenotrophomonas sp. Pemsol was isolated from a crude oil contaminated soil. The capability of this isolate to tolerate and degrade polycydic aromatic hydrocarbons (PAH) such as anthraquinone, biphenyl, naphthalene, phenanthrene, phenanthridine, and xylene was evaluated in Bushnell Hass medium containing PAHs as the sole carbon sources. The metabolites formed after 30-day degradation of naphthalene by Pemsol were analyzed using Fourier Transform Infra-red Spectroscopic (FTIR), Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). The genome of Pemsol was also sequenced and analyzed. Results. Anthraquinone, biphenyl, naphthalene, phenanthrene, and phenanthridine except xylene can be used as sole carbon sources for Pemsol's growth in Bushnell Hass medium. The degradation of naphthalene at a concentration of 1 mg/mL within 30 days was tested. A newly formed catechol peak and the disappearance of naphthalene peak detected on the UPLC-MS, and GC-MS analyses spectra respectively confirmed the complete degradation of naphthalene. Pemsol does not produce biosurfactant and neither bio-emulsify PAHs. The whole genome was sequenced and assembled into one scaffold with a length of 4,373,402 bp. A total of 145 genes involved in the degradation of PAHs were found in its genome, some of which are Pemsol-specific as compared with other 11 Stenotrophomonas genomes. Most specific genes are located on the genomic islands. Stenotrophomonas sp. Pemsol's possession of few genes that are associated with bio-emulsification gives the genetic basis for its inability to bio-emulsify PAH. A possible degradation pathway for naphthalene in Pemsol was proposed following the analysis of Pemsol's genome. ANI and GGDH analysis indicated that Pemsol is likely a new species of Stenotrophomonas. It is the first report on a complete genome sequence analysis of a PAH-degrading Stenotrophomonas. Stenotrophomonas sp. Pemsol possesses features that make it a good bacterium for genetic engineering and will be an excellent tool for the remediation of crude oil or PAH-contaminated soil.

引用

页数：25