Purification of glucose-6-phosphate dehydrogenase and glutathione reductase enzymes from the gill tissue of Lake Van fish and analyzing the effects of some chalcone derivatives on enzyme activities

被引：6

作者：

Kuzu, Muslum ^{[1
]}

Aslan, Abdulselam ^{[2
]}

Ahmed, Ishtiaq ^{[3
]}

Comakli, Veysel ^{[2
]}

Demirdag, Ramazan ^{[2
]}

Uzun, Naim ^{[1
]}

机构：

[1] Ibrahim Cecen Univ Agri, Fac Pharm, TR-04100 Agri, Turkey

[2] Ibrahim Cecen Univ Agri, Dept Nutr & Dietet, TR-04100 Agri, Turkey

[3] Karlsruhe Inst Technol, D-76021 Karlsruhe, Germany

来源：

FISH PHYSIOLOGY AND BIOCHEMISTRY | 2016年 / 42卷 / 02期

关键词：

Glucose-6-phosphate dehydrogenase; Glutathione reductase; Lake Van fish; Chalcone; Inhibition; CHALCALBURNUS-TARICHI; 6-PHOSPHOGLUCONATE DEHYDROGENASE; CYPRINIDAE; PALLAS; LIVER;

D O I：

10.1007/s10695-015-0153-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose-6-phosphate dehydrogenase (G6PD) and glutathione reductase (GR) are metabolically quite important enzymes. Within this study, these two enzymes were purified for the first time from the gills of Lake Van fish. In the purifying process, ammonium sulfate precipitation and 2',5'-ADP Sepharose 4B affinity column chromatography techniques for glucose-6-phosphate dehydrogenase, temperature degradation and 2',5'-ADP Sepharose 4B affinity column chromatography for glutathione reductase enzyme were used. The control of the enzyme purity and determination of molecular weight were done with sodium dodecyl sulfate polyacrylamide gel electrophoresis. K (M) and V (max) values were determined with Lineweaver-Burk plot. Besides, the effects of some chalcone derivatives on the purified enzymes were analyzed. For the ones showing inhibition effect, % activity-[I] figures were drawn and IC50 values were determined. K (i) value was calculated by using Cheng-Prusoff equation.

引用

页码：483 / 491

页数：9

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