Use of Raman optical tweezers for cell cycle analysis

被引：12

作者：

Ahlawat, Sunita ^{[1
]}

Chowdhury, Aniket ^{[1
]}

Uppal, Abha ^{[1
]}

Kumar, Nitin ^{[1
]}

Gupta, Pradeep Kumar ^{[1
]}

机构：

[1] Raja Ramanna Ctr Adv Technol, Laser Biomed Applicat & Instrumentat Div, Indore 452013, India

来源：

ANALYST | 2016年 / 141卷 / 04期

关键词：

SINGLE LIVING CELLS; TUMOR-CELLS; FLOW; SPECTROSCOPY; DNA; IDENTIFICATION; FLUORESCENCE; APOPTOSIS; LEUKEMIA; CULTURE;

D O I：

10.1039/c5an00971e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report the results of our investigations on the use of Raman optical tweezers for label free analysis of cells in different phases of their cell cycle. The studies performed on human colon adenocarcinoma (Colo-205) cells synchronized in G0/G1 and G2/M phases showed that the DNA Raman band at 783 cm(-1) in the Raman spectra of optically trapped cells can provide information about the DNA content in the nucleus of the cell without the need for the isolation of the nucleus. The histograms of intensity of this band among the cell populations were found to corroborate the results obtained from fluorescence image cytometry performed on DAPI stained cells.

引用

页码：1339 / 1346

页数：8

共 44 条

[1] Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry [J].

Camp, Charles H., Jr. ;

Yegnanarayanan, Siva ;

Eftekhar, Ali A. ;

Sridhar, Hamsa ;

Adibi, Ali .

OPTICS EXPRESS, 2009, 17 (25) :22879-22889

[2] Nondestructive identification of individual leukemia cells by laser trapping Raman spectroscopy [J].

Chan, James W. ;

Taylor, Douglas S. ;

Lane, Stephen M. ;

Zwerdling, Theodore ;

Tuscano, Joseph ;

Huser, Thomas .

ANALYTICAL CHEMISTRY, 2008, 80 (06) :2180-2187

[3] Recent advances in laser tweezers Raman spectroscopy (LTRS) for label-free analysis of single cells [J].

Chan, James W. .

JOURNAL OF BIOPHOTONICS, 2013, 6 (01) :36-48

[4] The Effect of Cell Fixation on the Discrimination of Normal and Leukemia Cells with Laser Tweezers Raman Spectroscopy [J].

Chan, James W. ;

Taylor, Douglas S. ;

Thompson, Deanna L. .

BIOPOLYMERS, 2009, 91 (02) :132-139

[5]

Darzynkiewicz Z., 1997, CURR PROTOCOL CYTOM, P752

[6]

Darzynkiewicz Z., 2010, CURR PROTOC CYTOM

[7]

Darzynkiewicz Z, 2010, ADV EXP MED BIOL, V676, P137

[8] Hemoglobin degradation in human erythrocytes with long-duration near-infrared laser exposure in Raman optical tweezers [J].

Dasgupta, Raktim ;

Ahlawat, Sunita ;

Verma, Ravi Shanker ;

Uppal, Abha ;

Gupta, Pradeep Kumar .

JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (05)

[9] ANALYSIS AND DISCRIMINATION OF NECROSIS AND APOPTOSIS (PROGRAMMED CELL-DEATH) BY MULTIPARAMETER FLOW-CYTOMETRY [J].

DIVE, C ;

GREGORY, CD ;

PHIPPS, DJ ;

EVANS, DL ;

MILNER, AE ;

WYLLIE, AH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1133 (03) :275-285

[10] Quartz microfluidic chip for tumour cell identification by Raman spectroscopy in combination with optical traps [J].

Dochow, Sebastian ;

Beleites, Claudia ;

Henkel, Thomas ;

Mayer, Guenter ;

Albert, Jens ;

Clement, Joachim ;

Krafft, Christoph ;

Popp, Juergen .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (08) :2743-2746

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