Heparin binding domain of insulin-like growth factor binding protein-5 stimulates mesangial cell migration

被引：57

作者：

Abrass, CK

Berfield, AK

Andress, DL

机构：

[1] Vet Affairs Puget Sound Hlth Care Syst, Dept Med, Div Nephrol, Seattle, WA 98108 USA

[2] Univ Washington, Sch Med, Seattle, WA 98108 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | 1997年 / 273卷 / 06期

关键词：

insulin-like growth factor I; glomerulus; chemotaxis; rat;

D O I：

10.1152/ajprenal.1997.273.6.F899

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Insulin-like growth factor I (IGF-I) binding protein-5 (IGFBP-5) is produced by mesangial cells (MCs) and likely functions to modulate glomerular IGF-I activity. Although IGFBP-6 may be inhibitory for IGF-stimulated MC activity, preliminary studies suggested that IGFBP-5 acts directly on MCs. To investigate this further, we evaluated the effects of IGFBP-5 on rat MC migration. We found that the carboxy-truncated fragment, IGFBP-5-(1-169), inhibited IGF-I-stimulated migration, but intact IGFBP-6 simulated migration when IGF-I was not present. Demonstration that (125)I-labeled IGFBP-5 directly binds to MCs further supports an independent role for IGFBP-5. Because heparin inhibited MC binding of (125)I-IGFBP-5, we tested the heparin binding peptide, IGFBP-5-(201-218), for stimulatory activity. IGFBP-5(201-218) stimulated MC migration, and this effect was inhibited by heparin. Because the disintegrin, kistrin, blocked IGF-I-induced migration but not migration induced by IGFBP-5-(201-218), the migratory induction mechanism for the two peptides is different. These data indicate that separate, specific regions of IGFBP-5 are responsible for interactive effects with IGF-I as well as direct effects on MC activity.

引用

页码：F899 / F906

页数：8

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