Bovine seminal ribonuclease attached to nanoparticles made of polylactic acid kills leukemia and lymphoma cell lines in vitro

被引:23
作者
Michaelis, M
Matousek, J
Vogel, JU
Slavik, T
Langer, K
Cinatl, J
Kreuter, J
Schwabe, D
Cinatl, J
机构
[1] Univ Frankfurt Klinikum, Inst Med Virol, D-60528 Frankfurt, Germany
[2] Acad Sci Czech Republ, Inst Anim Physiol & Genet, CR-27721 Libechov, Czech Republic
[3] Univ Frankfurt, Inst Pharmazeut Technol, Biozentrum Niederursel, D-60439 Frankfurt, Germany
[4] Univ Frankfurt Klinikum, Zentrum Kinderheilkunde, Abt Hamatol & Onkol, D-60590 Frankfurt, Germany
关键词
aspermatogenesis; BS-RNase; leukemia; lymphoma; nanoparticles; polylactic acid;
D O I
10.1097/00001813-200006000-00007
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Bovine seminal ribonuclease (BS-RNase) is a protein with a number of biological effects. It shows antitumoral, aspermatogenic, antiembryonic, immunosuppressive and antiviral properties. The cytotoxic effects appear to be specific for tumor cells as non-malignant cells seem to be unaffected in vitro. Unfortunately, the in vivo application of BS-RNase so far was successful only when it was administered intratumorally. Therefore, the objective of the present investigation was to improve the properties of BS-RNase by attachment to nanoparticles made of polylactic acid (PLA-NP) using an adsorption method. This preparation was tested in vitro against leukemia (MOLT-4) and lymphoma (H9) cell lines sensitive and resistant to cytarabine. No difference between the nanoparticle preparation and pure BS-RNase was found in these tests, To examine the in vivo effects, the preparations were tested for their aspermatogenic and antiembryonal efficacy compared to the pure BS-RNase as a rapid test for antitumoral activity. The aspermatogenic and antiembryonal effects were enhanced by the nanoparticle preparation, Consequently, BS-RNase loaded adsorptively to PLA-NP holds promise for the in vivo use as an antitumoral agent. Further research will investigate the efficacy of this preparations in an in vivo tumor model. [(C) 2000 Lippincott Williams & Wilkins.].
引用
收藏
页码:369 / 376
页数:8
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