Development of Microsphere-Based Multiplex Branched DNA Assay for Detection and Differentiation of Avian Influenza Virus Strains

被引：6

作者：

Cha, Wonhee ^{[2
,3
]}

Ma, Yunqing ^{[1
]}

Saif, Yehia Mo ^{[2
,3
]}

Lee, Chang-Won ^{[2
,3
]}

机构：

[1] Affymetrix Inc, Fremont, CA 94555 USA

[2] Ohio State Univ, Coll Vet Med, Dept Vet Prevent Med, Columbus, OH 43210 USA

[3] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, Wooster, OH 44691 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 07期

基金：

美国农业部;

关键词：

SIGNAL AMPLIFICATION; TESTS; BDNA; RNA; PCR; H5;

D O I：

10.1128/JCM.01979-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed and evaluated a multiplex branched DNA assay for the detection and subtyping of avian influenza (AI) virus strains. The assay successfully detected all 94 AI virus strains of 15 different hemagglutinin (HA) subtypes tested while simultaneously differentiating 24 North American H5, 11 Eurasian H5, and 11 H7 strains. Our study demonstrates for the first time that a branched DNA method can detect targets that show a great amount of sequence variation.

引用

页码：2575 / 2577

页数：3

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