Role of aldose reductase in TGF-β1-induced fibronectin synthesis in human mesangial cells

被引：16

作者：

Zhang, Yuejuan ^{[1
]}

Huang, Ping ^{[1
]}

Jiang, Tao ^{[1
]}

Zhao, Jingjing ^{[1
]}

Zhang, Nong ^{[1
]}

机构：

[1] Fudan Univ, Dept Pathol, Shanghai Med Coll, Shanghai 200032, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 06期

关键词：

Aldose reductase; Fibronectin; AP-1; Transforming growth factor-beta 1; GROWTH-FACTOR-BETA; TRANSGENIC MICE; RENAL-DISEASE; GLOMERULOSCLEROSIS; DEGRADATION; PROGRESSION; EXPRESSION; COLLAGEN; PATHWAY; KIDNEY;

D O I：

10.1007/s11033-009-9811-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Accumulation of glomerular extracellular matrix (ECM) may result in glomerulosclerosis. Several lines of evidence indicate a key role for transforming growth factor-beta 1 (TGF-beta 1) in glomerular ECM synthesis and degradation, such as fibronectin (FN). Aldose reductase (AR) was proven to be one of the TGF-beta 1 responsive genes in cultured rat mesangial cells using the SSH-PCR method and there were positive correlation between the AR and TGF-beta 1 in our previous studies. So we assumed that AR could regulate FN synthesis. In this study, we explored the role of AR in FN production and possible mechanism involved. The expression of AR, FN and c-Jun proteins were analyzed by Western blot and the activity of activator protein-1 (AP-1) was assessed by electrophoretic mobility shift assay (EMSA). Our results showed that AR could mediate the TGF-beta 1-induced FN production, which may associate with AP-1 activation.

引用

页码：2735 / 2742

页数：8

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