Bubble dynamics in double-stranded DNA

被引：74
作者：
Altan-Bonnet, G
Libchaber, A
Krichevsky, O
机构：
[1] Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA
[2] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel
来源：
PHYSICAL REVIEW LETTERS | 2003年 / 90卷 / 13期
关键词：
D O I：
10.1103/PhysRevLEtt.90.138101
中图分类号：
O4 [物理学];
学科分类号：
0702 ;
摘要：
We report the first measurement of the dynamics of bubble formation in double-stranded DNA. Fluctuations of fluorescence of a synthetic DNA construct, internally tagged with a fluorophore and a quencher, are monitored by fluorescence correlation spectroscopy. The relaxation dynamics follow a multistate relaxation kinetics, with a characteristic time scale of 50 mus. A simple model of bubble dynamics based on constant zipping-unzipping rates is proposed to account for our experimental data. The role of different secondary structures stabilizing the open bubble is tested.
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